Bacterial Toxin Research Citations

EAU Model

Incomplete Freund’s adjuvant (BD Difco, San Jose, CA, USA) containing 5 mg/mL Mycobacterium tuberculosis H37Ra was mixed with 2 mg/mL human interphotoreceptor retinoid-binding protein (IRBP)1-20 (GL Biochem, Shanghai, China) in a 1:1 volume ratio. The emulsion was injected into the back of each mouse, near the tail and two flanks. In addition, 200 ng of Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA, USA) was injected intraperitoneally into the immunized mice on days 0 and 2 after immunization.15,16

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… to one filled with the retrograde tracer Fluorogold (FG; 1%; Fluorochrome, Englewood, CO, USA) dissolved in 0.1 M sodium acetate buffer, or cholera toxin B subunit (CTb; 1%; List Biological Laboratories, Campbell, CA, USA) dissolved in 0.1 M PB, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

We purchased … Pertussis toxin was purchased from List Biological Laboratories (#180; Campbell, CA, USA), …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Experimental food allergy

Mice were sensitized intraperitoneally with 50 μg OVA (grade V; Sigma-Aldrich, Munich, Germany) plus 10 μg cholera toxin (CT; 100B; List Biological Laboratories, Los Angeles, CA, USA) in 150 mL phosphate-buffered saline (PBS) on days 0 and 14. Two weeks after the second sensitization, mice were challenged intragastrically with 100 mg OVA in 200 mL PBS 6 times within 2 weeks. Control mice were sensitized and challenged using only PBS. The rectal temperature was measured before and 30 minutes after the last oral OVA challenge. Mice showing profuse liquid stool within 60 minutes after the final challenge were recorded as diarrhea-positive. Intestinal tissue, spleen, and blood samples were collected from mice 1 day after the last challenge.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… 4+ T cells reactive against MOG were generated as follows. RFPxNurr77-GFP mice were immunized with 75 µg of MOG35-55 emulsified in complete Freund’s adjuvant (CFA) containing inactivated Mycobacterium tuberculosis H37RA (Difco) at a final concentration of 1mg/ml. Pertuxin toxin (PTX, 200ng, List Biological Laboratories) was injected on day 0 and day 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice were orally immunized with 10 µg of CT (List Biological Laboratories, Campbell, CA) and, when indicated, admixed with OVA at 10 mg per dose and dissolved in 0.5 ml of phosphate-buffered saline (PBS) containing 3% (w/v) NaHCO3 …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Where indicated, mice received 10 µg of cholera toxin (List Labs) by oral gavage. In some cases, mice were immunized intraperitoneally with 10 µg of peptides plus 20 µg …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Chronic EAE.

Six- to eighteen-week-old female C57BL/6 or DEREG Foxp3-DTR (diphtheria toxin [DT] receptor) mice were immunized with
50 mg MOG35 55 (AnaSpec) in CFA consisting of IFA (Difco Laboratories) and 5 mg/ml M. tuberculosis H37RA (Difco Laboratories). A total of 200 ng pertussis toxin (List Biological) was injected i.p. on days 0 and 2 p.i. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… emulsified in complete Freund’s adjuvant (CFA) (Sigma-Aldrich, St. Louis, MO, USA) mixed with 5.0 mg/mL Mycobacterium tuberculosis strain (MTB), and then intraperitonealy injected with 500 ng of pertussis toxin (List Biological, Campbell, CA, USA) as described previously …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… based on their ultrastructural level, we injected CTB (List Biological Laboratories, CO, USA) into the TA muscle. Retrograde labeling was observed at the light microscope …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae