Citations

Bacterial Toxin Research Citations

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5002 citations found

Lipopolysaccharide affects energy metabolism and elevates nicotinamide N-methyltransferase level in human aortic endothelial cells (HAEC)

St?pi?ska, O;Dymkowska, D;Mateuszuk, ?;Zab?ocki, K;

Product: ULTRA PURE LPS from Escherichia coli O111:B4

  • 2.1. Cell culture and treatment

    HAECs were purchased from Lonza. Cells were grown in EGM BulletKit®−2 (Lonza) at 37ºC in an atmosphere of 5% CO2 and 95% air. The culture medium was composed exactly as recommended by the supplier (Lonza). Confluent cells were exposed to 100 ng/ml LPS (Escherichia coli O111:B4, List Biological Laboratories, #421) for a period within 0.5 h to 24 h as indicated in figure legends. In all experiments, cells of the third passage were used.

    Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

AMPK-Nrf2 Signaling Pathway in Phrenic Motoneurons following Cervical Spinal Cord Injury

Michel-Flutot, P;Efthimiadi, L;Djerbal, L;Deramaudt, T;Bonay, M;Vinit, S;

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

  • 2.2. Animal Groups and Surgical Preparation

    In this study, 63 animals were used, divided into 9 groups: control (n = 4), Sham animals (at 1 h (n = 6), 1 day (n = 7), 3 days (n = 7), and 7 days (n = 7) post-surgery), and C2-injured animals (at 1 h (n = 8), 1 day (n = 8), 3 days (n = 8), and 7 days (n = 8) post-injury). None of the C2-injured animals died during or after the procedure, however one Sham animal died at 1 h due to a technical issue. All animals (except n = 4 of each group) received an intrapleural injection with the retrograde tracer Cholera toxin B fragment (CTB, List Biologicals, Campbell, CA, USA) to identify phrenic motor neurons in the cervical spinal cord, similar to that described previously [41,42,43,44]. Briefly, each rat was anaesthetized with isoflurane (Iso-vet, Piramal, Voorschoten, Netherlands; ~1.5% in O2), placed in the supine position on a surgical table while spontaneously breathing into a face mask. The lateral sides of the rib cage were shaved and gently palpated to identify the fifth intercostal space at the anterior axillary line. A sterilized 26G needle connected to a 50 μL Hamilton syringe was used to inject 25 μL of a 0.2% CTB (dissolved in sterile injectable saline) between the 5th and the 6th ribs into the pleural space on each side. …

    Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

IκBζ controls IL-17-triggered gene expression program in intestinal epithelial cells that restricts colonization of SFB and prevents Th17-associated pathologies

Yamazaki, S;Inohara, N;Ohmuraya, M;Tsuneoka, Y;Yagita, H;Katagiri, T;Nishina, T;Mikami, T;Funato, H;Araki, K;Nakano, H;

Product: LPS from Escherichia coli O111:B4

The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay

Nyström, S;Kendall, A;Adepu, S;Lindahl, A;Skiöldebrand, E;

Product: LPS from Escherichia coli O55:B5

  • 2.1. Chondrocyte isolation and culture

    … Samples were placed in sterile saline (0.9% NaCl) solution with gentamicin sulfate (50 mg/l) and amphotericin B (250 μg/ml). The cartilage samples were transported chilled (approx. 5 °C) to the laboratory. Isolation and expansion of chondrocytes were performed as previously described (Ley et al., 2011). Briefly, the chondrocytes were expanded to passage 1 and then seeded at 20,000 cells/cm2 in chondrogenic medium to maintain the phenotype. On day 4, cells were stimulated with LPS (10 ng/ml, Escherichia coli 055:B5; List Biological Laboratories, Campbell, CA, USA) or kept untreated (controls) for 24 h. Cells were grown to confluence and harvested on day 5 and immediately frozen and stored at −80 °C until further analyses.

    Product #203A – LPS from Escherichia coli O55:B5

Chemogenetic activation of noradrenergic A5 neurons increases blood pressure and visceral sympathetic activity in adult rats

Souza, GMPR;Stornetta, DS;Vitali, AJ;Wildner, H;Zeilhofer, HU;Campbell, JN;Abbott, SBG;

Product: Anti-Cholera Toxin B Subunit (Goat)

CpG 1018 adjuvant enhances Tdap immune responses against Bordetella pertussis in mice

DeJong, MA;Wolf, MA;Bitzer, GJ;Hall, JM;Sen-Kilic, E;Blake, JM;Petty, JE;Wong, TY;Barbier, M;Campbell, JD;Bevere, JR;Damron, FH;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

  • 4.6. Serological analysis of B. pertussis specific antibodies

    On the day of processing, a cardiac puncture was performed on each mouse. Approximately 1 mL of blood was removed from each mouse. The samples were centrifuged for 2 min at 14,000 × g. Serum was collected and stored at − 80 °C until analyses were performed. Serological responses specific to B. pertussis antigens were quantified by ELISA. High-binding microtiter plates were coated with PT (50 ng/well) (PT#180, LIST Biologicals), FHA (50 ng/well) (Enzo Life Sciences), or PRN (50 ng/well) (PRN#187, LIST Biologicals) as described in Boehm et al. [52]. …

    Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
    Product #187 – Pertactin from B. pertussis (69 kDa Protein)

Small sensory spinal lesions that affect hand function in monkeys greatly alter primary afferent and motor neuron connections in the cord

Fisher, KM;Garner, JP;Darian-Smith, C;

Product: Anti-Cholera Toxin B Subunit (Goat)

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation

Sasamoto, Y;Lee, CAA;Wilson, BJ;Buerger, F;Martin, G;Mishra, A;Kiritoshi, S;Tran, J;Gonzalez, G;Hildebrandt, F;Jo, VY;Lian, CG;Murphy, GF;Ksander, BR;Frank, MH;Frank, NY;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

  • Colony-forming assay

    Colony-forming assay (CFA) was performed as previously reported (Sasamoto et al., 2020). Briefly, limbal epithelial cells were seeded on the mitomycin C (MMC) (MilliporeSigma)-treated 3T3-J2 feeder cell layer at 500 cells per well on 6-well plates. The cells were cultured in keratinocyte culture medium (KCM) supplemented with 10 ng/mL KGF and 10 μM Y-27632. KCM was composed of DMEM without glutamine and Ham’s F-12 Nutrient Mix (Thermo Fisher Scientific) combined at 3:1 ratio, supplemented with 10% FBS, 0.4μg/ml hydrocortisone hydrogen succinate (MilliporeSigma), 2nM 3,3′,5-triiodo-l-thyronine sodium salt (MilliporeSigma), 1 nM cholera toxin (List Biological Laboratories, Campbell, CA), …

    Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Ex vivo and in vitro Monocyte Responses Do Not Reflect in vivo Immune Responses and Tolerance

Jansen, A;Bruse, N;Waalders, N;Gerretsen, J;Rijbroek, D;Pickkers, P;Kox, M;

Product: LIST™ HPT™ from Escherichia coli O113

  • Experimental Human Endotoxemia (IV LPS Administration)

    All experimental endotoxemia-related procedures were performed as described previously and were identical on both LPS challenge days (days 0 and 7) [19, 22]. In short, subjects were admitted to the research unit of the Radboud University Medical Center for 8 h. Subjects had to refrain from alcohol and caffeine (24 h) and food and drinks (12 h) prior to LPS administration. A radial artery catheter (BD Infusion Therapy Systems, Sandy, UT, USA) and antebrachial venous cannula were placed to allow serial blood sampling, hemodynamic monitoring, and administration of fluids and LPS, respectively. In the 45 min prior to LPS administration, hydration fluids (2.5% glucose/0.45% sodium chloride) were administered as a 1.5-L prehydration bolus to reduce the risk of vasovagal collapse [23] and thereafter at a rate of 150 mL/h for the remainder of the experiment. Directly after prehydration, a bodyweight-adjusted bolus dose of 1 ng/kg LPS (Escherichia coli-derived, Type O113, lot No. 94332B1; List Biological Laboratories, Campbell, CA, USA) was administered. Blood samples were serially obtained to construct time-concentration curves of circulating cytokines.

    Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113