Bacterial Toxin Research Citations

… A3V was injected into one side of the MLd in 19 chicks. Of these, 13 chicks were injected with the mixture of A3V-RSV-mCherry and 0.5% CTB (List Labs, Campbell, CA), and six chicks were injected with A3V-RSV-mCherry …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… The peptide emulsion was injected subcutaneously at 200 µg per mouse. Pertussis toxin (PTX; List Biological Labs, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… immunized with 100 µl of peptide emulsions on both sides of the flank followed by intraperitoneal injection (IP) of 400 ng pertussis toxin per mouse (List Biologicals …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE

… On days 0 and 2, mice were injected i.p. with 200 ng pertussis toxin (cat. no.180; List Biological Laboratories). Mice were evaluated for EAE symptoms every 2 d. The severity of EAE was analyzed according to the following clinical scoring system …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Lipopolysaccharide (LPS; List Biological Laboratories, #201, from Escherichia coli O111:B4 or #421, from Salmonella minnesota R595; store long term at -20°C) …

• Product #201 – LPS from Escherichia coli O111:B4
• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

EAE

Mice were immunized subcutaneously with 100 μg of myelin oligodendrocyte glycoprotein (MOG)35–55 peptide emulsified in CFA, which was made from IFA (Difco) plus Mycobacterium tuberculosis (Difco). Mice were injected intraperitoneally with 200 ng of pertussis toxin (PTX, List Biological Laboratories) on the day of immunization and 2 d later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents

Pertussis toxin (PTX) was obtained from List Biological Labs.

Induction of EAE

MOG35-55-induced EAE in C57BL/6 mice was established as previously described (11–13). In brief, the mice were immunized on day 0 with an intracutaneous injection of 150 mg of MOG35-55 emulsified in CFA-bearing M. tuberculosis strain H37Rv. Injection volume was set at 150 µl. Thereafter, each mouse received an additional 300 ng of PTX by intraperitoneal injection in 100 ml of phosphate-buffered saline (PBS) on day 0 and again on day 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Food allergy model

… For the peanut-induced systemic anaphylaxis model50, C3H/HeJ mice were intragastrically sensitized with 10 mg peanut extract (GREER Laboratories, USA) and 20 μg cholera toxin (List Biological Laboratories, USA) 4 times weekly. The animals were then orally boosted with 50 mg peanut extract and 20 μg cholera toxin twice every 2 weeks. At week 8,200 mg peanut extract was orally administered and rectal temperature was measured. In this model, IgETRAP was intraperitoneally injected the day before challenge of 200 mg peanut, and 5 × 109 cfu of B. longum was intragastrically administered to each mouse (5 × 109 cfu head−1) every day from week 5 to 8. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Cholera Toxin List Biological laboratory 100B …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction and assessment of EAE

Acute EAE was induced and assessed as previously described (Kang et al, 2010). Briefly, acute EAE was induced by subcutaneous immunization with 300 μg of the MOG35-55 peptide (Met-Glu-Val-Gly-Trp-Tyr-Arg-Ser-Pro-Phe-Ser-Arg-Val-Val-His-Leu-Tyr-Arg-Asn-Gly-Lys, Sangon Biotech Co.) in CFA containing 5 mg/ml heat-killed H37Ra strain of Mycobacterium tuberculosis (Chondrex, Inc) in the back region and both sides of the vertebrae. And the immunized mice were i.v. injected with pertussis toxin (List Biological Laboratories, Inc.) at a dose of 250 ng per mouse in PBS on the day of immunization and once more 48 h after the first injection. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)