Bacterial Toxin Research Citations

Animals and induction of EAE

The EAE was actively induced in conventional inbred female C57Bl/6J mice (9-13 weeks old)
by immunization with myelin oligodendrocyte glycoprotein (MOG) 35-55 peptide (Prospec,
Rehovot, Israel) and complete Freund’s adjuvant (CFA) containing Mycobacterium
tuberculosis H37Ra (Sigma-Aldrich, Prague, Czech Republic). The MOG/CFA emulsion was
prepared by connecting two glass syringes to a 3-way connector and passing the solutions
through the connector. The emulsion was injected subcutaneously in two 50 μl doses. In total,
100 μg of MOG peptide per mouse was administered. To facilitate the transfer of lymphocytes
into the CNS, 300 ng of pertussis toxin (List Biologicals, Campbell, California, USA) in 200
μl of phosphate buffer saline (PBS) was injected intraperitoneally two hours and two days after
the EAE induction [14].

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Drugs
… PTX was obtained from List Biological Laboratories. …

Split luciferase and complex BRET assays

HEK293T cells seeded in six-well plates were cotransfected with plasmids encoding smBiT-, LgBiT-, and mKO-tagged components as previously described (12). Twenty-four hours later, the cells were plated onto clear bottom, white-walled 96-well plates at 50,000 to 100,000 cells per well in BRET medium [clear MEM (Gibco) supplemented with 2% FBS, 10 mM Hepes, 1× GlutaMAX, and 1% penicillin/streptomycin/amphotericin B (Gibco)]. Select cells were then treated overnight with PTX at a final concentration of 200 ng/ml. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAU

Uveitis was induced as described [34] with modification. In brief, mice were immunized with 500 µg of human IRBP1–20 peptide (Anaspec, Fremont, CA) emulsified in Complete Freund’s Adjuvant (CFA; Sigma) and supplemented with Mycobacterium tuberculosis (strain H37Ra; BD Difco, Detroit, MI) to a final concentration 2.5 mg/ml. On the day of immunization, mice were also i.p. injected with 1 µg Bordetella pertussis toxin (PTX; List Biological Laboratories, Campbell, CA).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin List Biological Laboratories via Quadratech (Epsom, UK) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… To test whether (R)-BPO-27 treatment reduces CT-dependent diarrheal disease symptoms, groups of 3–5-day-old wild type (CD-1) mice were mock treated with the vehicle control containing DMSO in Luria broth (LB), purified CT (List Biological Laboratories, Campbell, CA, USA), purified CT mixed with (R)-BPO-27, or with (R)-BPO-27 alone (Figure 1A). A second dose of vehicle or (R)-BPO-27 was administered 6 h after the initial challenge. Mice treated with CT alone had statistically significant elevated FA ratios (diarrheal disease) at 22 h (Figure 1B). By contrast, mice treated with CT mixed with (R)-BPO-27 developed significantly less diarrheal disease than mice treated with CT alone (Figure 1B). Importantly, there was no significant difference in diarrheal disease between mice treated with CT and (R)-BPO-27 and mice treated with (R)-BPO-27 alone or with the vehicle control. We conclude that two doses of (R)-BPO-27 protect neonatal mice from diarrheal disease induced by oral-gastric administration of CT. These results are consistent with the findings of Verkman and colleagues who studied the ability of (R)-BPO-27 to block fluid accumulation induced by CT and STa in isolated (i.e., ligated) intestinal segments of adult mice [23]. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Pertactin (cat. no. 187), pertussis toxin mutant (cat. no. 184), and fimbriae 2/3(cat. no. 186) were purchased from List Laboratories, USA. …

• Product #186 – Fimbriae 2/3 from B. pertussis
• Product #187 – Pertactin from B. pertussis (69 kDa Protein)
• Product #184 – Pertussis Toxin Mutant

… Recombinant protective antigen and edema factor were obtained from List Biologicals (Campbell, CA, USA) …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Product #178A – Anthrax Edema Factor (EF), Recombinant from B. anthracis

… Staphylococcal enterotoxin type B (SEB) toxin List Biological Laboratories CAT# 122 …

… Chicken polyclonal anti-TcdB List Biological Laboratories 754A …

… For double immunostaining with mCherry and CTB, sections were incubated overnight with a mixture of rabbit anti-DsRed antibody (1:1000 dilution; Clontech) and goat anti-CTB antibody (1:10,000 dilution; List Biological Laboratories, Campbell, CA, USA; Cat. # 703; RRID:AB_10013220) in PBS-XD …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)