Bacterial Toxin Research Citations

… After a location was found from which a compound movement resembling the p-Synergy could be evoked with low currents, the stimulating pipette was removed from the brain, cleaned with deionized water, and filled with 1% cholera toxin (CT); List Biological Laboratories …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Animal studies and treatments

… Mice were treated with 0.5µg DT or CRM197 (List Biological) i.p. on day 0 only or on days 0, 4, 7, and 10. For in vivo competition assays, mice were treated on day 0 with 0.125-0.5µg DT and/or CRM197. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
• Product #149A – Diphtheria Toxin CRM197, Mutant

… Anthrax Lethal Factor (LF-A) (#171E) and Anthrax Protective Antigen (PA) (#169L) were obtained from List Biological Laboratory (California, USA). …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

… On the same day as immunization and 2 days later, mice were also injected IP with 300 ng of pertussis toxin (List Biological Labs, Campbell, California, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and assessment of EAE

Active EAE was induced as previously described (38). EAE induction was performed with age- and sex-matched mice. Briefly, mice were injected subcutaneously with the MOG35–55 peptide (200 μg) in Complete Freund’s Adjuvant (CFA) containing heat-killed Mycobacterium tuberculosis (5 mg/ml; H37Ra strain, BD Diagnostics). On the day of immunization and 48 hours later, mice were injected intraperitoneally with Pertussis toxin (200 ng per mouse; List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Microinjection of CTB Into the LR3 and LR8 on the Opposite Side of Hindlimb

Six rats were used for the injection of CTB into the LR3 and LR8 on the opposite sides of hindlimbs. Considering the application of
the CTB on the peripheral site only produced neural labeling on the same side of injection, bilateral injections were performed in
this study. This is also in order to reduce the number of animals used. Under the anesthesia with 2% isoflurane, 2 µl of 1% CTB
solution (List Biological Labs, Inc., Campbell, CA, United States) was injected subcutaneously and muscularly with Hamilton
micro-syringe (Hamilton Company, Reno, NV, United States) into the right side of LR3 and the left side of LR8, respectively. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

ELISPOT assay

ELISPOT was performed to enumerate cholera toxin and LPS specific plasmablast present in the PBMC samples. 96-well ELISPOT assay filter plates (Millipore) were coated overnight at 4°C with CTB (List Labs #104), …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Cell-rounding assay.

This assay was used to measure toxin activity, as C. difficile toxins cause cell rounding of tissue cultures, and was performed as described previously (43) with slight modifications. Briefly, Vero cells (monkey kidney epithelial cells) from ATCC were aliquoted in 24-well plates at a density of 103 cells/ml and incubated overnight at 37°C in a CO2 incubator. Cells were treated with serially diluted concentrations of ebselen (ranging from 125 ng/ml to 128 μg/ml) for 30 min, followed by the addition of TcdB (List Biologicals Laboratories, Inc.) to a final concentration of 20 ng/ml. The plates were incubated at 37°C in a CO2 incubator for 2 h and then observed with phase-contrast microscope (Nikon Diaphot 200/300 inverted microscope) with counting from 5 views per well.

• Product #155 – Toxin B from Clostridium difficile

… Control animals received subcutaneous injections lacking MOG33-55 peptide. At the time of immunization and 48?h later, mice received intraperitoneal injections with 500 ng pertussis toxin (List Biologicals, cat. no. 180)

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Sections were the incubated with goat anti-CTB (List Biological Laboratories Cat #703, 1:10,000) …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)