4664 total record number 37 records this year

Structure-guided design of a potent Clostridiodes difficile toxin A inhibitor

Hussack, G;Rossotti, MA;van Faassen, H;Murase, T;Eugenio, L;Schrag, JD;Ng, KK;Tanha, J;

Crystal structures of camelid heavy-chain antibody variable domains (VHHs) bound to
fragments of the combined repetitive oligopeptides domain of Clostridioides difficile
toxin A (TcdA) reveal that the C-terminus of VHH A20 was located 30 Å away from
the N-terminus of VHH A26. Based on this observation, we generated a biparatopic
fusion protein with A20 at the N-terminus, followed by a (GS)6 linker and A26 at the
C-terminus. This A20-A26 fusion protein shows an improvement in binding affinity
and a dramatic increase in TcdA neutralization potency (>330-fold [IC50]; ≥2,700-
fold [IC99]) when compared to the unfused A20 and A26 VHHs. A20-A26 also shows
much higher binding affinity and neutralization potency when compared to a series
of control antibody constructs that include fusions of two A20 VHHs, fusions of two
A26 VHHs, a biparatopic fusion with A26 at the N-terminus and A20 at the C-terminus
(A26-A20), and actoxumab. In particular, A20-A26 displays a 310-fold (IC50) to
29,000-fold (IC99) higher neutralization potency than A26-A20. Size-exclusion
chromatography-multiangle light scattering (SEC-MALS) analyses further reveal that
A20-A26 binds to TcdA with 1:1 stoichiometry and simultaneous engagement of
both A20 and A26 epitopes as expected based on the biparatopic design inspired
by the crystal structures of TcdA bound to A20 and A26. In contrast, the control
constructs show varied and heterogeneous binding modes. These results highlight
the importance of molecular geometric constraints in generating highly potent
antibody-based reagents capable of exploiting the simultaneous binding of more
than one paratope to an antigen.