Detection of Stx2 in the E. coli O157:H7 culture supernatants and in fecal samples from mice, described below, was carried out by ELISA, as previously described.38 Briefly, camelid anti-Stx antibody (List Biological Laboratories, Campbell, CA) was coated on a white MaxiSorp nunc plate (Thermo Fisher Scientific, Rockford, IL) and incubated overnight at 4°C. ...
... For immunofluorescence staining, tissue sections were stained with camelid anti-Stx1 and Stx2(List Biological Laboratories, Campbell, CA, USA) followed by goat anti-llama:FITC (Invitrogen). ...