Additional information
| Weight | 1 lbs |
|---|---|
| Dimensions | 10 × 10 × 10 in |
| Destination |
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LPS from Escherichia coli K12, D31m4 (Re)
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Products are for research purposes only and are
not for use in humans or as diagnostic agents.
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$210.00
#302 (5 mg)
Form
lyophilized powder
Storage temp
2-8°C
Lysophosphatidylcholine Acetyltransferase 2 (LPCAT2) Influences the Gene Expression of the Lipopolysaccharide Receptor Complex in Infected RAW264.7 Macrophages, Depending on the E. coli Lipopolysaccharide Serotype
Poloamina, Alrammah, Abate et al
Biology (Basel) (2024) 13 (5) DOI: 10.3390/biology13050314
Poloamina, Alrammah, Abate et al
Biology (Basel) (2024) 13 (5) DOI: 10.3390/biology13050314
Cited by 1
Snippet:
2. Materials and Methods
... Semi-rough (Rc) LPS—E. coli J5 and Rough (Re) LPS—E. coli K12 D32m4—was purchased from List Biological Laboratories, ...
2.3. Bacterial Infection
LPS was resuspended in LAL reagent water (<0.005 EU/mL endotoxin levels). Adding 100 ng/mL of either lipopolysaccharide serotype to a flask containing RAW264.7 cells stimulated the desired inflammatory response for 6 h, after which the culture medium was removed, and the cells were collected for analysis. ...
LPCAT2 Regulates CD14 Expression During Macrophage Inflammatory Response to E. coli O111:B4
Poloamina, Abate, Fejer et al
bioRxiv (2023)
Poloamina, Abate, Fejer et al
bioRxiv (2023)
Snippet:
Materials and Methods
... Rc LPS - E. coli J5, List Biological Laboratories, 301; Re LPS - E. coli K12 D32m4, List Biological Laboratories, 302]. ...
Chitinase-Induced Airway Hyperreactivity and Inflammation in a Mouse Model of Nonallergic Asthma
Weber-Chrysochoou, Darcan-Nicolaisen, Wohlgensinger et al
Int Arch Allergy Immunol (2021)
Weber-Chrysochoou, Darcan-Nicolaisen, Wohlgensinger et al
Int Arch Allergy Immunol (2021)
Cited by 3
Snippet:
Cell Stimulation and Total RNA Extraction
Cells grew in 6-well plates at 37°C with 5% CO2 until they were 80% confluent. The cells were stimulated in a serum-free medium for 6 h with LPS 100 ng/mL (Escherichia coli K12, List Biological Laboratories, Campbell, CA, USA), ...
Regulation of The Expression and Lysine Acetylation of Pro-Inflammatory Molecules By Lipid-Modifying Enzyme (LPCAT2) in RAW264. 7 Cells
Poloamina
Thesis (2021)
Poloamina
Thesis (2021)
Snippet:
Cell Stimulation
RAW264.7 macrophages were stimulated with 100ng/ml E. coli O111:B4 [Sigma-Aldrich, L260], J5 [List Biological Laboratories, 301], and K12, D31m4 [List Biological Laboratories, 302] LPS for various durations to analyse the effect of LPCAT2 and RNF19B silencing on the expression ...
Interaction between the retrotrapezoid nucleus and the parafacial respiratory group to regulate active expiration and sympathetic activity in rats
Zoccal, Silva, Barnett et al
Am. J. Physiol. Lung Cell Mol. Physiol. (2018)
Zoccal, Silva, Barnett et al
Am. J. Physiol. Lung Cell Mol. Physiol. (2018)
Snippet:
... current pulses, 7 s duration every 7 s for 10 min) of the retrograde tracer cholera toxin subunit b - CTb (low salt, 1% in distilled water, List Biological Laboratories, Campbell, USA). CTb was detected using a goat anti-CTb antibody (1:2000, List Biological ...
Transcutaneous immunization with pneumococcal surface protein A in mice
Nagano, Kawabata, Sugita et al
Laryngoscope (2017)
Nagano, Kawabata, Sugita et al
Laryngoscope (2017)
Snippet:
Methods:
C57BL/6 mice were transcutaneously immunized with 1µg of PspA and 2µg of cholera toxin (CT) six times at weekly intervals and compared with transcutaneously treated controls (PBS alone/PspA alone/CT alone). ...
... Cholera toxin (CT) (List Biological, Campbell
Interferon-γ-dependent control of Anaplasma phagocytophilum by murine neutrophil granulocytes
Gussmann, Kirschnek, von Loewenich
Parasit Vectors (2017) 10 (1), 329 DOI: 10.1186/s13071-017-2274-6
Gussmann, Kirschnek, von Loewenich
Parasit Vectors (2017) 10 (1), 329 DOI: 10.1186/s13071-017-2274-6
Snippet:
Experimental design:
Host-cell free A. phagocytophilum obtained from 3 107 Hoxb8 neutrophils with an infection rate of 90% was used to infect 1.2 107 Hoxb8 neutrophils (differentiated for 4 days) in 6 ml medium. ... Depending on the experiment, cells were stimulated with 10 ng/ml or 200 ng/ml Escherichia coli K12 D31m4 (Re) LPS (List Biologicals, Campbell, CA, USA) or ...
Multivalent chromosomal expression of the Clostridium botulinum serotype A neurotoxin heavy chain antigen and Bacillus anthracis protective antigen in Lactobacillus acidophilus
O'Flaherty, Klaenhammer
Appl. Environ. Microbiol. (2016)
O'Flaherty, Klaenhammer
Appl. Environ. Microbiol. (2016)
Snippet:
Western Blot Assays:
...Primary antibody was then added in 10 ml of 5% NFDM in TBST...and for PA, anti-protective antigen from B. anthracis, goat, 1:5,000, List Biological Laboratories, Campbell, CA) and incubated overnight at 4C. ...
Intestinal mucus affinity and biological activity of an orally administered antibacterial and anti-inflammatory peptide
Dupont, Kaconis, Yang et al
Gut (2015) 64 (2), 222-32 DOI: 10.1136/gutjnl-2014-307150
Dupont, Kaconis, Yang et al
Gut (2015) 64 (2), 222-32 DOI: 10.1136/gutjnl-2014-307150
Cited by 8
Snippet:
LPS from Escherichia coli K12 D31m4 (Re) was obtained from List Biological Laboratories (Campbell, California, USA).
Calpastatin is upregulated in non-immune neuronal cells via toll-like receptor 2 (TLR2) pathways by lipid-containing agonists
Vaisid, Kosower
Biochim. Biophys. Acta (2013) 1833 (10), 2369-77 DOI: 10.1016/j.bbamcr.2013.06.006
Vaisid, Kosower
Biochim. Biophys. Acta (2013) 1833 (10), 2369-77 DOI: 10.1016/j.bbamcr.2013.06.006
Cited by 6
Snippet:
SH-SY5Y cell culture, and cell treatment with TLR agonists, with Ca2 +/ionomycin, and with TLR pathways inhibitors:
... The TLR agonists used were: ... lipopolysaccharide LPS (E. coli K12, D31m4(Re)) (List Biological Laboratories Inc., California, USA) (stock solution of 1 mg/ml) was added at a final concentration of 100 ng/ml; ...
Showing 1-10 of 12 papers.
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