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#550 (100 nmoles)
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Transduction of mouse astroglial cells with bONE-GO sensor and BRET measurements
... Kinetic traces are represented as change in BRET after subtraction of the baseline signal measured for 30 s before GPCR stimulation [ΔBRET·103 (baseline)]. Where indicated in the figures or figure legends, cells expressing Gαi bONE-GO WT were treated overnight with 0.1 μg/ml pertussis toxin (List Biological Labs, cat#179A). For the concentration-response curve presented in Fig. 7C, the average [ΔBRET·103 (baseline)] of the “Buffer†condition was first subtracted from all traces, and then the area-under-curve (AUC) was calculated in Prism (GraphPad) for each trace, followed by curve fit to a 3-parameter sigmoidal equation.
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
... Biotinylated recombinant PA83 (List Biological Labs, Campbell, CA) was bound ...
Experimental SNAP-Etide assay:
Testing of compounds identified through the virtual screen was performed using a commercially available SNAP-Etide substrate (List Biological Inc., CA) as employed in prior work, ...
... Pertussis toxin (List Biological Laboratories, Cambell, CA) treatment of macrophages was carried out as described (Holian, 1986). ...
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
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