Bacterial Toxin Research Citations

Induction and assessment of EAE

EAE was induced by subcutaneous injection with 200 μg of mouse MOG35–55 peptide (Prespec) emulsified in complete Freund’s adjuvant containing heat-killed Mycobacterium tuberculosis H37RA (BD Difco) on day 0. Pertussis toxin (200 ng; List Biological Laboratories) was intravenously injected on the day of immunization and 2 days after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

STAR★Methods
Key resources table

Lipopolysaccharide from Salmonella Minnesota List Labs Cat#R595

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… Pertussis toxin (150 ng per mouse, list labs) was given i.v. on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

MATERIALS AND METHODS.

4.1 Reagent.

… Purified LPS was purchased from List Biological Lab. …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

Cell cultures

… Established cancer cell lines were cultured according to ATCC recommendations. DT22 cells and BM-MSCs were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10%fetal bovine serum (FBS). MCF10A cells were cultured in DMEM/Ham’s F12 medium supplemented with 5% horse serum (Gibco BRL, Waltham, MA), insulin (10 μg/ml; Wisent Bioproducts, Saint-Jean Baptiste, QC), hydrocortisone (500 ng/ml; Sigma–Aldrich, St. Louis, MO), epidermal growth factor (EGF) (20 ng/ml; R&D, Minneapolis, MN), and cholera toxin (100 ng/ml; List Biological Laboratories, Campbell, CA).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Then, adherent macrophages underwent further incubation in serum-free RPMI1640 at 37°C for 2h and subsequent treatment with LPS (List Biological Laboratories, Escherichia coli 055:B5), …

• Product #203A – LPS from Escherichia coli O55:B5

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction of myelin-reactive Th17 cells ex vivo and the adoptive transfer EAE model

… To induce EAE by adoptive transfer, CD4+ T cells were isolated from the MOG35-55-specific Th17 cell culture with the CD4+ T Cell Isolation Kit 5 days after stimulation. CD4+YFP+ T cells further purified on a FACSAriaTM III were adoptively transferred into Tcrb−/− mice via tail vein injection (5 × 106 cells/injection). One day after transfer, recipient mice were immunized with MOG35-55 peptide in CFA and then given an intraperitoneal injection of pertussis toxin (PTX, 500 ng/injection) (List Biological Laboratories, Campbell, CA, USA)12. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction

Male MeCP2 WT and MUT mice, 9 weeks old, were anesthetized via i.p. with a mixture of xylazine and ketamine (16 mg/kg and 80 mg/kg respectively) and immunized subcutaneously at the right and left flanks with 200 μl of an emulsion containing 200 μg of myelin oligodendrocyte glycoprotein peptide (MOG35–55, NH2-MEVGWYRSPFSRVVHLYRNGK-COOH; synthesized at the Johns Hopkins University Synthesis & Sequencing Core Facility, Baltimore, MD, USA). The peptide was dissolved in sterile water at 2 mg/ml, mixed at a 1:1 ratio with complete Freund’s adjuvant (CFA, Sigma-Aldrich Co., St. Louis, MO, USA), supplemented with 4 mg/ml of Mycobacterium tuberculosis. Pertussis toxin (200 ng List Labs, USA) was dissolved in 100 μl of phosphate-buffered saline (PBS) and injected i.p. the same day of the immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For immunofluorescence staining, tissue sections were stained with camelid anti-Stx1 and Stx2 (List Biological Laboratories, Campbell, CA, USA) followed by goat anti-llama:FITC (Invitrogen). …