Bacterial Toxin Research Citations

… Diphtheria toxoid (60 µg) (List Biological Laboratories, Campbell, CA) and a negative control of recombinant Schistosoma mansoni glutathione-S-transferase (GST) (15 µg) (Moss et al., 2004) were each coupled to 1.25 × 10 7 beads in a 1 ml volume of buffer containing …

• Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae

… Adjuvanted formulations contained cholera toxin B (List Biological, cat #104) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Pertussis toxin List Biological Laboratories Cat# 180…

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Then ultrapure LPS (0.01 µg/mL, List Laboratories, Campbell, CA) was added to the wells in the continued presence or absence of ERK1/2 inhibitor (1 µM) …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

2.2 Myelin oligodendrocyte glycoprotein (MOG)‐induced EAE model

Under anesthesia with isoflurane, female C57BL/6 mice were immunized in the rear flanks by subcutaneous injection (s.c.) of 150 μg MOG35‐55 peptide (Biotrend Chemicals, Zurich, Switzerland) emulsified 1:1 in Complete Freund’s Adjuvant (CFA) (Chondrex, Redmond, WA, USA) containing 4 mg/mL Mycobacterium tuberculosis. On days 0 and 2 postimmunization, mice were injected intraperitoneally (i.p.) with 400 ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA). Vehicle or ACT‐1004‐1239 were given from day 0 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction and Clinical Scoring

EAE was induced after a minimum of 30 days post TAM administration by subcutaneous immunization at two sites of the chest with an emulsion that contained 100 μg MOG p35-55 (Genemed Synthesis) and Complete Freund’s Adjuvant containing 200 μg of heat-killed Mycobacterium tuberculosis (H37RA, Difco Laboratories). Mice were also injected with Bordetella pertussis toxin (PTX) (List Biologicals, dose used was lot-dependent, but varied between 100 and 250 ng) on days 0 and 2 following immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Stereotaxic Surgery

… For conventional retrograde tracing, rats were given unilateral microinjections of 100 nl of 2% Fluoro-Gold (FG, Fluorochrome, Denver, CO, USA) into unilateral NAc core (AP +1.8, ML +2.6, DV –7.3 from bregma, 6°) and 100 nl of 0.2% cholera toxin subunit b (CTB, List Biological Laboratories, Campbell, CA, USA) into RMTg (AP –7.2, ML +1.9, DV –7.6 from dura, 10°). FG was used only in NAc because it tended to cause toxicity in RMTg, evident by circling of animals after unilateral injection. Rats were sacrificed at least 1 week after injections. …

Tissue Processing

… For FG and CTB tracing, free-floating sections were incubated overnight in goat anti-CTB (1:50 K, List Biological Labs Cat# 703, RRID: AB_10013220) in PBS with 0.25% Triton X-100 …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Mice were immunized subcutaneously at four injection sites with a total of 200 µl of emulsified incomplete Freund’s adjuvant supplemented with … and received intraperitoneal injections of 200 ng pertussis toxin (PTX; List Biological Laboratories) at the time of immunization and 48 h later …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Flow cytometry of T cells

… For ICS, we stimulated cells with overlapping peptide pools (15 mers overlapping by 11 amino acids) targeting the S1 or S2 domains of spike glycoprotein, N, or E proteins (JPT Peptide Technologies). The S1 pool spans the N-terminal amino acid residues (1–643 amino acids, 158 peptides) of spike glycoprotein, while the S2 pool spans the C-terminal amino acid residues (633–1273 amino acids, 157 peptides). Each peptide pool was reconstituted with 40 or 50 μL of pure DMSO (Sigma-Aldrich) and then diluted with PBS for a final concentration of 100 μg/mL in 16% DMSO/84% PBS or 20% DMSO/80% PBS. PBMC were plated at a density of up to 1 × 106 cells/well in a 96-well U-bottom plate and stimulated with 1 μg/mL of each peptide in the pool, or 0.25 μg/mL Staphylococcal Enterotoxin Type B (SEB) (List Biological Laboratories Inc.) …

Mouse tracheal epithelial cell harvest and growth conditions

Mice of each genotype (WT, SP-A-/-, SP-A2 223Q, and SP-A2 223K) were euthanized with Urethane and tracheas were removed and placed in Ham’s F-12 media on ice. … The media used to culture the mouse tracheal epithelial cells (MTECs) consisted of Ham’s F-12 media supplemented with 250 ng/ml amphotericin B solution (Hyclone), 20 ng/ml cholera toxin (LIST biological), 104 μg/ml bovine pituitary extract (Lonza), 5 μg/ml insulin (Sigma), 5 μg/ml apo-transferrin (Sigma), 0.1uM dexamethasone (Sigma), 5ng/ml mouse epidermal growth factor (Sigma), 0.01 μM retinol (Sigma), 20 U/ml nystatin (Sigma), 50 μg/ml gentamicin. During the growth and expansion phase of culture, this media was further supplemented with 10% FBS. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae