Bacterial Toxin Research Citations

… An intraperitoneal injection of 300ng Pertussis toxin (List Biological Laboratories, Cedarlane, Canada) was administered at the time of induction and 48h later. Control Page 31. 19 animals were treated with CFA (1.5mg/ml) and Pertussis toxin…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… To induce EAE, … All animals received an intraperitoneal (i.p.) injection of pertussis toxin (300 ng, List Biological Laboratories, Cedarlane, Canada), administered at the time of induction and 48 hours later in order to facilitate an immune response …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Lipopolysaccharide from Salmonella Minnesota List Labs Cat#R595

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae list labs Cat# 150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… 200 ng Pertussis-Toxin (List Biological Laboratories, Hornby, Ontario, Kanada; Kat-Nr. #181) in 500 µl steriler phosphatgepufferter Salzlösung (phosphate-buffered saline = PBS) (Dulbecco´s PBS; Sigma-Aldrich) …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Cell Stimulation

RAW264.7 macrophages were stimulated with 100ng/ml E. coli O111:B4 [Sigma-Aldrich, L260], J5 [List Biological Laboratories, 301], and K12, D31m4 [List Biological Laboratories, 302] LPS for various durations to analyse the effect of LPCAT2 and RNF19B silencing on the expression …

• Product #301 – LPS from Escherichia coli J5 (Rc)
• Product #302 – LPS from Escherichia coli K12, D31m4 (Re)

4.1. Drugs and chemicals

… Pertussis toxin was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials

… chicken polyclonal IgY against TcdB (#754A) from List Biological Labs …

2.2. Probe conjugations

… The number of oligonucleotides that successfully modified onto the DNP was quantified using the Qubit® ssDNA Assay Kit (Invitrogen by Thermo Fisher Scientific, USA). BNP probes were fabricated as follow: the DNA-modified DNPs (10 nM, 0.2 mL) were reacted with excess GBP-tagged BoNT/A LC (0.3 μM, 0.2 mL) for 0.5 h at 37 °C. Then it was collected and resuspended with reaction buffer (50 mM HEPES, pH 7.4, 500 mM NaCl, 0.05% TWEEN20) for later usage. And the amount of BoNT/A LC conjugated onto the BNP was estimated using the SNAPtide (FITC/DABCYL, List Biological Laboratories, Campbell, CA).

• Product #521 – SNAPtide® Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin

4.1.2. Mouse Model

EoP was induced in C57BL/6j mouse pups using a combination of hypoxia-ischemia (HI) and systemic inflammation, as described recently by our group [32], which is an adaptation of the model originally described by from Shen, Plane, and Deng [85]. At P5 HI was induced by permanent unilateral occlusion of the right common carotid artery under isoflurane anesthesia (5–10 min; 5% induction, 3% maintenance with flow O2: air 1:1). After a 75-min recovery period, pups were subjected to hypoxia (6% O2) during 35 min under temperature-controlled conditions (35.8–36.0 °C). Subsequently, animals received an i.p. injection of 1mg/kg LPS (List Biological Laboratories, Campbell, CA, USA), dissolved in sterile 0.9% NaCl. …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/