Citations

Elisa

TcdA and TcdB toxins of the infected tree shrew stools were detected by ELISA method. The fresh fecal samples were collected after C. difficile infection for one week of all animals. Feces were suspended to 0.5 g/ml concentration by using PBS buffer (pH 7.4). The fecal suspensions were coated in 96-well plate at 4 °C overnight, and then washed with PBST (PBS pH 7.4 + 0.05% tween-20). The plate was blocked with 5% skim milk at 37 °C for 2 h. The polyclonal antibodies of TcdA and TcdB (List Biological Laboratories, Chicken IgY, 1: 1000 dilution) were used for first hybridization; then Goat anti-Chicken IgY Secondary Antibody, HRP (Invitrogen, 1: 5000 dilution) was used. ELISA-TMB Chromogenic Reagent kit (Sangon, China) was used to determine the absorbance at 450 nm. Pure toxin A and toxin B (List Biological Laboratories) were used as positive controls, and the standard curves were determined at 20 ng/ml, 2 ng/ml, 0.2 ng/ml, 0.02 ng/ml and 0.002 ng/ml both for TcdA and TcdB.

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

Oral Sensitization and Challenge of Mice

After 2 weeks, mice were sensitized weekly for 5 times with 10 µg cholera toxin (List Biological Laboratories, Campbell, CA, USA) or 20 mg whey (sweet whey protein concentrate 60 [sWPC60];

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunohistochemistry

… CTB (goat, 1:4,000, List Biological Laboratories),…

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… For basophil depletion, WT and basophil-depleted mice were treated with 0.375μg of diphtheria toxin (catalog no. 150; LIST Biological Laboratories) intraperitoneally every other day; mice were killed 37?days post-N. brasiliensis infection …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Induction of EAU and clinical scoring

To induce EAU, 300 μg Inter photoreceptor binding protein (IRBP) residues 651–670 (Hanhong group, Shanghai, China) were emulsified with an equal volume of complete Freund’s adjuvant (CFA) (Sigma-Aldrich, USA) containing 2.5 mg/ml Mycobacterium tuberculosis strain 37RA (Sigma-Aldrich, USA). Pertussis toxin (0.5 μg) (List Biological Laboratories, USA) was administered intraperitoneally on the day of immunization and 24 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The EAE model was provided by “Salari Institute of Cognitive and Behavioral Disorders (SICBD)”. …All mice received two doses of 0.3 μg pertussis toxin (List Biological Lab, Campbell, CA, USA) through theintraperitoneal (i.p.) injection about two hours and forty-eight hoursafter immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The medulla and cervical spinal cord were sectioned at 40 μm. Every second section from the hypoglossal region of the medulla and from C3-C5 within the cervical spinal cord was stained with an anti-cholera toxin antibody (List-Biologicals, 703) …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Two intraperitoneal injections of 400 ng pertussis toxin (List Biological Laboratories, Denver, CO, USA) were given 0 and 48 hours later …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice were immunized on days 0, 7, and 14 by intraperitoneal injection of 100 µl of injectable grade PBS containing: PBS only (control groups), 20 µg LLO T in the presence or in the absence of 1 µg cholera toxin (List Biological Laboratories, Inc, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Induction of EAM was performed as described previously [1, 19]. Briefly, the hind soles of rats were injected with 200 μl of human cardiac myosin (2 mg/ml) mixed with an equal volume of complete Freund’s adjuvant supplemented with 5 mg/ml Mycobacterium tuberculosis H37RA (Difco, Detroit, MI, USA). Experimental animals were intraperitoneally administered with 500 ng of pertussis toxin (List Biological Laboratories, Inc., Campbell, CA, USA) on the day of immunization day 0 and 2 days post-immunization (D2PI). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)