Bacterial Toxin Research Citations

STAR★methods
KEY RESOURCES TABLE
REAGENT OR RESOURCE Source Identifier

… Ultra-pure LPS List Biological Laboratories Cat# 225 …

ELISA
Mouse peritoneal macrophages were primed with ultra-pure LPS (3 ng/ml; List Biological Lab) for 3 hr and were then stimulated with crystals for 3 hr at 37°C. IL-1β in cell culture supernatants (sup.) was measured by ELISA (R&D Systems).

• Product #225 – LPS from Salmonella typhimurium

Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae List Biological Laboratories, INC. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Pertussis Toxin List Biological Technologies Cat# 180 …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

4-hydroxytamoxifen, diphtheria toxin, and tamoxifen treatment

… Diphtheria toxin (DT, List Biological Laboratories, 150) was dissolved in PBS. 1µg (first dose) or 613 250 ng (subsequent doses) was injected intraperitoneally for each mouse. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Pertussis toxin (salt free; 250 ng; List Biological Laboratories, Inc.) was injected intraperitoneally on day 0 and day 2 post-immunization. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

FACS analysis

Differentiated hiPSCs were analyzed by FACS as described previously (Hayashi et al., 2017, 2018). Cells were then dissociated using StemPro™ Accutase™ Cell Dissociation Reagent (Thermo Fisher Scientific) and resuspended in cold KCM medium (DMEM without glutamine and Nutrient Mixture F-12 Ham [3:1; Thermo Fisher Scientific] supplemented with 5% FBS [Japan Bio Serum, Hiroshima, Japan], 0.4 μg/mL hydrocortisone succinate [Wako], 2 nM 3,3′,5-triiodo-l-thyronine sodium salt [MP Biomedicals, Santa Ana, CA, USA], 1 nM cholera toxin [List Biological Laboratory, Campbell, CA, USA], 2.25 μg/mL bovine transferrin HOLO form [Thermo Fisher Scientific], 2 mM l-glutamine, 0.5% insulin transferrin selenium solution [Thermo Fisher Scientific], and 1% penicillin-streptomycin solution). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

A total of 1 µL of 1% CTB (List Biological Labs, Campbell, CA, USA) solution was slowly injected under the epineurium of the sciatic nerve on the left leg using a 10 µL micro-syringe (Hamilton Company). Following injection, the incision was sutured closed using …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

ELISA

Schistosoma mansoni egg antigens (SEA) and tetanus and diphtheria-specific IgG1 endpoint titers were determined by enzyme-Linked immunosorbent assay (ELISA) using the mAb X56 (BD) and Immulon 4HB plates (Thermo Fisher Scientific) as previously described [21]. Briefly, plates were coated with 2μg/mL tetanus (List Labs), diphtheria (Sigma), or SEA. The following morning plates were blocked with 1% milk and incubated with serial dilutions of primary antibody, followed by incubation with anti-mouse IgG1 ads-HRP antibody (Southern Biotech) and ABTS substrate. Plates were read at 405 nm at room temperature on a BioTek plate reader.

• Product #190 – Tetanus Toxin from Clostridium tetani

… For the detection of any GM1-binding CTB molecules irrespective of the length or sequence of C-terminal extension, a previously established method using a goat polyclonal anti-CTB antiserum (List Biological Laboratories, Campbell, CA) was employed …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

2.8 Adoptive transfer

For passive EAE, mice were euthanized 10 days after MOG35–55 immunization, and spleen and lymph nodes were collected as previously described [27]. Single cells were incubated for 3 days in the presence of 25 µg/mL MOG35–55, 10 ng/mL recombinant murine IL-23 (R&D, 1887-ML-010), and 2 ng/mL IL-2 (R&D, 402-ML-020) at 1 × 107 cells/mL. CD4+ T cells were purified by CD4+ T cell isolation kit and 1 × 106 cells per mouse were transferred via intravenous injection. Pertussis toxin (200 ng/mouse, List Biological Laboratories, 181) was injected intraperitoneally (i.p.) on days 0 and 2. EAE score was assessed by daily recording using a 0–5 scale.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)