Citations

EAE model

… C57BL/6J mice were anesthetized by sevoflurane, and then subcutaneously injected with 100 μL MOG35-55 (3 mg/mL)/IFA (H37RA 4 mg/mL) (catalog: 231141, Difco Laboratories, USA) into the two sides of the upper part of the back on day 0. The mice were intraperitoneally (i.p.) injected with 50 μL of pertussis toxin (PTX) (0.5 μg/100 μL) (catalog: 180, List Biological Labs, Inc.). Forty-eight hours after the injection, the mice were injected with 50 μL of PTX for a second time. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Induction of EAE.

EAE was induced as previously described (15). Female SJL/J mice (8–12 weeks) were obtained from Janvier SAS. A total of n = 35 mice in 6 cohorts were used in the study. Mice were immunized s.c. with 250 μg PLP139–151 (Pepceuticals Ltd.) and 800 μg mycobacterium tuberculosis H37Ra (Difco), and they were administered 200 ng of pertussis toxin (List Biological Laboratories) on d0 and d2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

STAR★Methods

… Diphteria Toxin (DTX) List Biological Lab Cat# 150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Chemicals.

… and botulinum toxin B was purchased from List Biological Labs (CA, USA). …

STAR★Methods
Key Resources Table

DT List Biological Laboratories Cat. #150

Microglia depletion and dissociation

CD11bDTR mice were anesthetized by isoflurane and 1 μL DT solution (20 ng/μl) or saline was injected into the lateral ventricle of the mice. At 12 h after DT-injection, mice were subjected to 3 times FS to test seizure latency and then decapitated for Iba-1 immunostaining, or mice were anesthetized by isoflurane for flow cytometry. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Then, the above emulsion was injected subcutaneously (day 1). Pertussis toxin (PTX; List Biological Labs, Campbell, CA, USA) (300 ng) was intraperitoneally administered on the first and third days after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For canonical inflammasome stimulations, overnight (ON) IFNγ‐primed BMDMs were then prestimulated with PAM3CSK4 (InvivoGen, 100 ng/ml) for 4 h to induce pro‐IL1β expression. Then, Nigericin (NLRP3 activator, 40 μM, InvivoGen), dA:dT (AIM2 activator, 1 μg/ml, InvivoGen), TcdB toxin (PYRIN inducer, 0.05 μg/ml, List Biological Laboratories) or flagellin (NLRC4 trigger, 1 μg/ml, InvivoGen) was used to stimulate various canonical inflammasomes. Both flagellin and poly(dA:dT) were transfected into cells using FuGeneHD (Promega) transfection reagent in Opti‐MEM culture medium. When specified, P. aeruginosa (PAO1) and S. Tm strains (SL1344) (MOI 5) were used to trigger NLRC4 inflammasome response …

• Product #155 – Toxin B from Clostridium difficile

Induction of myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis

To induce EAE, all mice were immunized with MOG35–55 peptide (MEVGWYRSPFSRVVHLYRNGK; CelTek Bioscience, Nashville, TN) emulsified in an equal volume of complete Freund’s adjuvant (CFA; BD Difco, Franklin Lakes, NJ) and pertussis toxin (Ptx; List Biological Laboratories, Campbell, CA). Mice were anesthetized with isoflurane, and MOG35–55:CFA (100 μL; 3 mg/mL) was subcutaneously injected on each hind flank (200 μL total/mouse) on day 0. Ptx (200 μL; 2.5 μg/mL) was administered intraperitoneally (IP) on day 0 and day 2 post-MOG immunization (dpm). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… While stabilizing the animal’s back, the rib cage was palpated in order to identify the fifth intercostal space at the anterior axillary line. Using sterile conditions and appropriate antisepsis, a 50 µl Hamilton syringe was used to inject 15–20 μl of a 0.25% Cholera Toxin B Subunit (CTB, Choleragenoid) from Vibrio cholera (List Biological Labs) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… On days 0 and 2 after transfer, recipient mice were given 200 and 400 ng of pertussis toxin (List Biologicals), respectively. Mice were scored according to the previously established criteria (27) by an investigator blinded to the mouse genotype/treatment. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)