Citations

Induction, treatment, and clinical evaluation of EAE

… Mice were anaesthetized with isourane and 120 μl of emulsion was injected subcutaneously on each flank (200 μl total/mouse) on day 0. In addition, 500 μl of pertussis toxin (1μg.ml -1; List Biological Laboratories, Campbell, CA) was injected into the tail vein on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… lipopolysaccharide (LPS) from Salmonella Minnesota R595 (Re) was obtained from List Biological Laboratories (Campbell …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Animals and deafening protocol

… Diptheria toxin (DT; List Biologicals Lab. Inc. cat. #150) was dissolved in saline, aliquoted and stored at − 20 °C until use. Repeat freeze–thaw cycles were avoided. A single dose of 20 ng/g DT was administered intra-muscularly to adult mice. Mice receiving adenovirus (Ad) inoculation surgery, were given DT immediately after the surgery. High-calorie diet gel (DietGel 76A, ClearH20) and an extra bottle of water were provided for the 2 following weeks. Body weight and general health metrics were monitored every day for a week after DT administration.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… For Treg depletion, diphtheria toxin (DT; List Biologicals, Campbell, CA) was administered intraperitoneally 2 days and 1 day before intratracheal instillation of bacteria and then on days 1, 3 after inoculation. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagent:

Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae List Biological Laboratories Cat# 150

Tumor Associated Macrophage Co-Injection Experiments

Foxp3DTR-GFP donor mice were injected as mentioned above with either PyMT or EO771 cells, and their tumors were allowed to grow until approximately 90mm3. Half of the donors received two doses of 50 μg/kg of diphtheria toxin (DT) in two consecutive days, and primary tumors from both groups were harvested four days after initial DT treatment.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Experimental Autoimmune encephalomyelitis

… Pertussis toxin (120 ng) (List Biological Laboratories) diluted in PBS was administered i.v. on days 0 and 2 post-immunization. The clinical severity of EAE was scored by an observer who was unaware of mouse genotypes, using a grading scale of 0–5 as previously described.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… (2) DTR mice with DT injection at 5 weeks of age (DT group, N=32 animals). Mice in both groups received a single intramuscular injection of DT (15 or 20 ng/g) The DT (List Biological Laboratories, Inc., Campbell CA. Product #150) was reconstituted to a concentration of 2 mg/ml, aliquoted, and stored at 20°C. The aliquots were thawed and diluted in saline 1:200 just prior to the injection …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… On the day of immunization and 2 days later, mice received an intraperitoneal (IP) injection of 500 ng pertussis toxin (List Biological, CA, USA). Mice were weighed and neurologic function was evaluated daily by researchers blinded to treatment history …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For each mouse, injections were performed at both flanks and nape of the neck. Subsequently, pertussis toxin (List Biological Lab, USA) was injected intraperitoneally, 2 h, and 24 h after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunization & challenge

BALB/c mice were immunized with 5 μg of OMPOT with cholera toxin (List Biological Laboratories, Inc., Campbell, CA, USA) through i.n. route (Thermo Scientific) 3 times at 2-wk interval. Mice injected with PBS were used as a negative control. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae