Bacterial Toxin Research Citations

… Diptheria toxin List Biological Laboratories Cat# 150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Cell Stimulation and Total RNA Extraction

Cells grew in 6-well plates at 37°C with 5% CO2 until they were 80% confluent. The cells were stimulated in a serum-free medium for 6 h with LPS 100 ng/mL (Escherichia coli K12, List Biological Laboratories, Campbell, CA, USA), …

• Product #302 – LPS from Escherichia coli K12, D31m4 (Re)

2.2. Induction of EAN and evaluation of clinical score

P0180–199 peptide (SSKRGRQTPVLYAMLDHSRS, > 98% purity) was synthesized by Ontores Biotech (Hangzhou, China). Pertussis toxin (PTX) was purchased from List Biological Laboratories (CA, USA). Freund’s incomplete adjuvant and Heat killed Mycobacterium tuberculosis (strain H37RA) were purchased from Becton Dickinson (BD) Difco (MI, USA). In brief, mice were anesthetized with isoflurane each time before immunization. PTX solution of 1.6 μg/mL 250 μL, 1.2 μg/mL 250 μL, and 1.2 μg/mL 250 μL were given respectively on Day -1, 1, 3 intraperitoneally. P0180–199 peptide of 2 mg/mL 25 μL was emulsified in Freud’s complete adjuvant (20 mg/mL solution of Mycobacterium tuberculosis in 25 μL Freund’s incomplete adjuvant). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Three hours after nucleofection, cells were stimulated with 1 µg/mL of TcdA (List Biological Laboratories, Campbell, CA, USA) …

• Product #152C – Toxin A from Clostridium difficile

Immunofluorescence Histochemical Staining of DMV and Analysis

Triple immunofluorescence staining of CTb, VGLUT1, and ChAT was conducted for CTb-injected rats. Sections containing DMV was processed for CTb, VGLUT1, and ChAT triple-immunofluorescence staining. Briefly, the sections were incubated at room temperature with a mixture of goat anti-CTb Ig (1:2,000; List Biological, Campbell, CA, USA), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Reagents

… and pertussis toxin was obtained from List Biological Laboratories (Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Multiplex luminex immunoassays

Antigen-specific antibody responses were measured through a modified multiplex Luminex assay as previously reported (22, 45). Pertussis (PTM, PT, inactivated PT), tetanus, and diphtheria proteins, PRN, FHA, FIM2/3, adenylate cyclase toxin (ACT), lipooligosaccharide (LOS), tetanus toxoid (T), and diphtheria toxoid (d), were purchased from List Biological Laboratory (Campbell) and …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #186 – Fimbriae 2/3 from B. pertussis
• Product #187 – Pertactin from B. pertussis (69 kDa Protein)
• Product #184 – Pertussis Toxin Mutant
• Product #188L – Adenylate Cyclase Toxin, Recombinant from B. pertussis
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
• Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae
• Product #190 – Tetanus Toxin from Clostridium tetani
• Product #191 – Tetanus Toxoid from Clostridium tetani

… Azide-free cholera toxin (CT: #100B) from Vibrio cholerae was obtained from List Biological Laboratories (Campbell, CA, U.S.A.) and used as the adjuvant. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

STAR★Methods
Key Resources Table

Pertussis toxin List Biological Laboratories Cat# 180(LB)

EAE induction and monitoring

For adoptive transfer of effector T cells, 5×106 Th17 or Th1 1C6 T cells were injected i.v., in cold PBS into NOD.Scid recipient mice, which additionally received 200 ng of pertussis toxin (List Biological Labs) i.p. on d0 and d2 post-injection. FCG B6 x NOD F1 mice were actively immunized with 200 μg MOG[35-55] in 200 μL of incomplete Freund’s adjuvant supplemented with 500 μg M. tuberculosis extract, and additionally received 200 ng pertussis toxin (List Biological Labs) on d0 and d2. Th1 and Th17 cells generated from FCG B6 × 1C6 mice were adoptively transferred to NOD.Scid at 2 × 106 cells/recipient. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

STAR★Methods
KEY RESOURCES TABLE
REAGENT or RESOURCE SOURCE IDENTIFIER

Chemicals, peptides, and recombinant proteins

Lethal factor List Biologicals Cat#172B

Protective antigen List Biologicals Cat#171E

Activation of BMDMs and human macrophages

All cells were grown, maintained and activated at 37°C and 5% CO2 in a water-jacketed incubator. 1 × 106 WT or Casp1/11 DKO murine macrophages were plated per 12-well in DMEM, 10% (v/v) FCS (DMEM complete medium) one day prior to stimulation. All BMDMs were primed with 200 ng/mL LPS from Salmonella Typhimurium for 2 h or left untreated and subsequently washed three times with serum- and phenol red-free DMEM supplemented with 2 mM L-glutamine (DMEM minimal medium). For the time course experiment (Dataset 1), cells were preincubated with 0 or 100 μM glyburide (#NBP2-30141, Novus Biologicals) for 15-30min before 2 mM ATP (#7699, Sigma) or 10 μM nigericin (N1495, Invitrogen) were added for 15, 30, 45, and 60 min. Lethal factor and protective antigen were added at 1ug/ml. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis‘
• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis