Bacterial Toxin Research Citations

2.1 EAE induction and drug delivery

… Pertussis toxin (List Biological labs Inc., Campbell, CA) was administered via intraperitoneal (ip) injection at day 0 (75 ng per mouse) and day 2 (200 ng per mouse) after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell culture and gene expression analysis

Cell culture plates (48-well) were coated overnight with PN, PN Fr., or other indicated allergens at 50 µg/ml in carbonate-bicarbonate buffer (0.05M, pH 9.6, Millipore-Sigma) and washed once with PBS, before mDC or other cell types were added (2-5×105 cells/well) in StemSpan medium. The controls lipopolysaccharide (LPS, 100 ng/ml; Millipore-Sigma) and cholera toxin (CT, 1 µg/ml; List Biological Laboratories, Campbell, CA) were used in solution instead of coated. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

2.2. Experimental Autoimmune Uveitis (EAU) Induction

EAU was induced by subcutaneous injection of interphotoreceptor retinoid-binding protein peptide 1–20 (IRBP; [Homo sapiens] H2N-GPTHLFQPSLVLDMAKVLLD-OH, New England Peptide, Gardner, MA, USA) 500 μg per mouse emulsified in CFA containing 3.3 mg/mL of heat-killed Mycobacterium tuberculosis H37Ra (Difco, Franklin Lakes, NJ, USA) immediately followed by intraperitoneal application of pertussis toxin (PTx; List Biological Laboratories, Inc., Campbell, CA, USA) 1.2 μg, as previously reported [36].

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

STAR★Methods
Key resources table

Pertactin from B. Pertussis (69 kDa Protein) List Biological Laboratories CAT # 187

Luminex

A multiplexed Luminex assay was used to determine relative titer of antigen-specific isotypes, subclasses, and FcR binding, as previously described (Brown et al., 2017). For this assay, the following antigens were used: SARS-CoV-2 RBD (kindly provided by Aaron Schmidt), SARS-CoV-2 S (kindly provided by Eric Fischer), SARS-CoV-2 N (Aalto Bio Reagents). SARS-CoV-2 S1 (Sino Biological), SARS-CoV-2 S2 (Sino Biological), pertussis pertactin (List Reagents) and a mix of HA A/Michigan/45/2015 (H1N1), HA A/Singapore/INFIMH-16-0019/2016 (H3N2), B/Phuket/3073/2013 (Immunetech). …

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

… Following overnight incubation in primary antibody (goat anti-CTB; List Biological Science, Campbell, CA) and extensive washing, sections were incubated in secondary antibody …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

2.5. Mice and EAE Induction of Experimental Autoimmune Encephalomyelitis (EAE)

MBP-TCR/DR2a Tg mice were immunized s.c. with 200 μg of SLPA in Complete Freund’s Adjuvant (CFA) (Difco Laboratories, Detroit, MI, USA) on day 0 and 200 ng of pertussis toxin (List Biologicals, St. Louis, MO, USA) was administered i.p. on days 0 and 2 for the induction of EAE. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Local delivery of compounds or viral particles on the sciatic nerve

For local compound delivery, 2.5 µl of each compound or its corresponding vehicle (saline or dimethyl sulfoxide (DMSO)) were slowly poured onto the nerve with a pipette to avoid damage on the nerve and allowed to penetrate the tissue for several minutes prior performing the injury or the sham procedure before suturing the wound. For retrograde tracing and transport and experiments, 3 µl of Dextran-584 (Life Technology) or 1% CTB (List Biological Laboratories) were injected into the nerve prior to dorsal column axotomy or sciatic nerve crush. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Pertussis toxin administration

Pertussis toxin (List Biological laboratories, Campbell, CA) was dissolved in distilled-deionized water
(DDW) followed by dilution with phosphate buffer saline (PBS) under sterile conditions. And PT was
administrated intraperitoneally at a dosage of 250 ng or 500 ng (1ng/ml) per mouse immediately after
surgery. The same volume PBS was given in the vehicle group.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.1. Materials

… Mono-phosphoryl lipid A (MPLA) and di-phosphoryl lipid A (DPLA), both derived from Salmonella minnesota R595, were purchased from List Biological Laboratories (Campbell, CA, USA, product #401 and product #304, respectively). …

2.2. In Vitro Studies: Effects of Mono- and Di-Phosphoryl Lipid A on Macrophages and Hepatic Stellate Cells

We examined the effects of wild-type LPS (which served as positive control), di-phosphoryl lipid A, and mono-phosphoryl lipid A on mouse RAW264.7 macrophages (ECACC, Porton Down, Wiltshire, UK) and in cultures of primary rat hepatic stellate cells (rHSCs), isolated from normal rat livers. rHSC (200,000 cells/mL) or RAW cells (100,000 cells/mL) were incubated for 24 h with wild-type LPS from E. coli (100 ng/mL) or DPLA (100 ng/mL) or MPLA (100 ng/mL), both from S. minnesota R595. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)
• Product #401 – LIPID A monophosphoryl from Salmonella minnesota R595

… Seven days later disease was induced (day 0) by administering … 200 ng of pertussis toxin (List Biological Laboratories), given twice on consecutive days. The health of the animals was recorded for 1 month. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)