Bacterial Toxin Research Citations

… Pertussis Toxin List Biologicals Cat#179A …

G-protein live-cell BRET measurements

…For endpoint measurements shown in Figure 1—figure supplement 1, data is presented as raw BRET ratios (535 nm luminescence/460 nm luminescence) of unstimulated cells. For Figure 2—figure supplement 1, PTX treatments consisted of overnight incubations with 0.2 µg/ml of the toxin. …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… assay (ELISA) protocol (15) with a few modifications. Briefly, 96-well enzyme immunoassay (EIA) plates were coated with 100ml of purified C. difficile TcdA (List Laboratories; catalog no. 152C) at 1mg/ml in 0.05 M sodium bicarbonate …

• Product #152C – Toxin A from Clostridium difficile

… Adherent cells were exposed to C. difficile toxin B (List labs, Campbell CA) …

• Product #155 – Toxin B from Clostridium difficile

…For cholera toxin B subunit (CTB, List Biological Laboratories) treatment, … Pam3CSK4 (InvivoGen) primed cells were stimulated with 20 mg/ml CTB plus …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE model establishment:

… An intraperitoneal injection of 200 ng of pertussis toxin was administered on day 2. The mice were examined daily for clinical signs of disease and evaluated as previously described …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and Evaluation of EAE

… On days 0 and 2, immunized mice received an intraperitoneal injection of 200 ng pertussis toxin (PTX, Cat: 181, Lot: 181238A1, List Biological Labs, CA, USA). …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Reagents

… LPS from E. coli O111:B4 was purchased from List Biological Laboratories (Campbell, CA, USA). …

• Product #201 – LPS from Escherichia coli O111:B4

… On days 0 and 2, 100 ng/mouse pertussis toxin (List Biological Laboratories) was given by ip injection. Mice were monitored and weighed daily until day 28 of the experiment. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… overnight at 4°C on a shaking platform in either polyclonal rabbit anti-FG primary antibody (1:50,000; Cat# Fluoro-gold, RRID:AB_2314408; Fluorochrome) or polyclonal goat anti-CTB primary antibody (1:10,000; Cat# 703, RRID:AB_10013220; List Biological Laboratories …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Mice were then injected i.p. with 60 ng of pertussis toxin (List Biological Laboratories, 179A) in PBS at 2 and 24 h after emulsion injection …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)