Citations

EAE induction and treatment

The EAE model was induced as previously described19. Mice were anaesthetized and subcutaneously immunized with 200 μl of 100 μg myelin oligodendrocyte glycoprotein (MOG35–55, Wuhan Haode Peptide, China) in incomplete Freund’s adjuvant (Sigma Aldrich, USA) containing 200 μg mycobacterium tuberculosis (strain H37Ra; Difco, USA) on day 0. Pertussis toxin (PTX; List Biologicals, USA) (200 ng) was administered intraperitoneally (i.p.) on day 0 and 2. EAE scores were assessed daily for clinical signs of EAE mice in a blinded fashion as previously described20. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials
… LPS derived from Salmonella Minnesota R595 (Re) was purchased from List Biological Laboratories, Inc. (Campbell, CA) and used for proB and proCE activation assays. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… AT-EAE was induced as previously described [6]. In brief, 2D2/TCRMOG mice were immunized with 200 µg of MOG3555 (MEVGWYRSPFSRVVHLYRNGK; Genscript, RP10245) emulsified in CFA (BD Difco, 263810) and treated with 200 ng of pertussis toxin (List Biological Laboratories, 179B) i.p. on the day of immunization …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Chemicals, Peptides, and Recombinant Proteins

Protective antigen (PA), recombinant from Bacillus anthracis List Biological Laboratories 171C

Stimulation assays

… For the stimulation of the NLRC4 inflammasome, human macrophages were primed with 200 ng ml-1 LPS for 3 hours before 2 μg ml-1 LFn-PrgI and 0.5 μg ml-1 PA (from Bacillus anthracis) were added to the culture medium for 2 hours (Zhao et al., 2011).

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

EAE Induction and Clinical Evaluation

EAE was induced as previously described [7,8,9]. Briefly, mice were immunized by the subcutaneous injection of 200 μL of a mixture of recombinant myelin oligodendrocyte glycoprotein 35-55 (MOG35-55, AnaSpec, Fremont, CA, USA) in a mixture of incomplete Freund’s adjuvant (IFA, Sigma-Aldrich, St. Louis, MO, USA) and Mycobacterium tuberculosis H37RaA (Difco Laboratories, Detroit, MI, USA). Pertussis toxin (PT, List Biological Laboratories, Campbell, CA, USA) was intraperitoneally injected at a dose of 400 ng on post-immunization days 0 and 2. A booster injection of MOG35-55 was given on day 7. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice were also injected intraperitoneally with 300 ng Pertussis toxin (PT; List Biological Laboratories) in 200 L PBS (final concentration 1.5 g/mL) on the day of immunization and 2 d later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… This was followed by an intraperitoneal (I.P.) injection of 250 ng of Bordetella pertussis toxin (List Biological Laboratories Inc.) in PBS at day 0 and day 2 post-immunization. Donor mice were sacrificed day 10 post-immunization and spleens and lymph nodes were harvested …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice were immunized weekly for 1, 6, or 12 weeks by oral gavage with ground blanched peanut (Western Mixers Produce & Nuts), roasted and unsalted peanut (Whole Foods Market), or wheat flour (Bobs Red Mill Company) or OVA (Sigma) at 5 mg food antigen, with or without 10 µg of CT (List Biologicals, lot nos. 10165A1 and 10167A2) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Table 2
Injected tracers used in this study

Cholera toxin subunit B CTb List Biological Laboratories Product #104 (Spangler 1992) 0.1% solution in dH20

Stereotaxic Injections

Mice were anesthetized with isoflurane (0.5–2.0%) and placed in a stereotactic frame (Kopf 1900 or 940). We made a midline incision and retracted the skin to expose the bregma and the skull over the brain. Through a pulled glass micropipette (20–30–μm inner diameter), we injected Fluorogold (FG), cholera toxin subunit B (CTb), or an AAV (Table 2).

Table 4
Antisera used in this study

Cholera toxin B (CTB) Purified choleragenoid, the B subunit of cholera toxin List Biological, goat polyclonal, cat. #703, lot #7032A9, RRID: AB_10013220 1:10 000

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

B Cell Enrichment and Culture

… Cells were maintained in 5% CO2 at 37°C. B cells were stimulated with LPS 0111:B4 (10 μg/ml, List Biological Laboratories, Campbell, CA, United States), …

• Product #201 – LPS from Escherichia coli O111:B4