Bacterial Toxin Research Citations

Lipopolysaccharides

We used the phenol–water method described by Westphal [25] to extract and purify the LPSs and cores from E. coli and Salmonella. The strains of E. coli that were used to obtain the LPSs and cores were as follows: … the core Ra came from S. minnesota (List Biological Laboratories, Campbell, C A, USA). The LPSs and cores were treated with DNase, RNase, and proteinase K. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

EAE

… Pertussis toxin (200 ng, ip)(List Biological Laboratories) was also injected at the same time as MOG(35-55) as well as 2 days later …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Anthrax Protective Antigen List Biologicals Ref: 771B…

Antigens, Peptides and Co-Stimulants

Purified genetically-detoxified Pertussis Toxin (PT; R9K, E129A; Limulus Amebocyte Lysate activity < 20 EU/mg) was purchased from LIST Biological laboratories Inc. (Campbell, CA, USA) and stored at 500 µg/mL at −20 °C.

Whole Blood Stimulation

… Different parallel stimulation conditions were performed, i.e., incubation in the presence of heat-inactivated PT (5 μg/mL), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and treatment

The EAE model was induced as previously described19. Mice were anaesthetized and subcutaneously immunized with 200 μl of 100 μg myelin oligodendrocyte glycoprotein (MOG35–55, Wuhan Haode Peptide, China) in incomplete Freund’s adjuvant (Sigma Aldrich, USA) containing 200 μg mycobacterium tuberculosis (strain H37Ra; Difco, USA) on day 0. Pertussis toxin (PTX; List Biologicals, USA) (200 ng) was administered intraperitoneally (i.p.) on day 0 and 2. EAE scores were assessed daily for clinical signs of EAE mice in a blinded fashion as previously described20. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials
… LPS derived from Salmonella Minnesota R595 (Re) was purchased from List Biological Laboratories, Inc. (Campbell, CA) and used for proB and proCE activation assays. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… AT-EAE was induced as previously described [6]. In brief, 2D2/TCRMOG mice were immunized with 200 µg of MOG3555 (MEVGWYRSPFSRVVHLYRNGK; Genscript, RP10245) emulsified in CFA (BD Difco, 263810) and treated with 200 ng of pertussis toxin (List Biological Laboratories, 179B) i.p. on the day of immunization …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Chemicals, Peptides, and Recombinant Proteins

Protective antigen (PA), recombinant from Bacillus anthracis List Biological Laboratories 171C

Stimulation assays

… For the stimulation of the NLRC4 inflammasome, human macrophages were primed with 200 ng ml-1 LPS for 3 hours before 2 μg ml-1 LFn-PrgI and 0.5 μg ml-1 PA (from Bacillus anthracis) were added to the culture medium for 2 hours (Zhao et al., 2011).

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

EAE Induction and Clinical Evaluation

EAE was induced as previously described [7,8,9]. Briefly, mice were immunized by the subcutaneous injection of 200 μL of a mixture of recombinant myelin oligodendrocyte glycoprotein 35-55 (MOG35-55, AnaSpec, Fremont, CA, USA) in a mixture of incomplete Freund’s adjuvant (IFA, Sigma-Aldrich, St. Louis, MO, USA) and Mycobacterium tuberculosis H37RaA (Difco Laboratories, Detroit, MI, USA). Pertussis toxin (PT, List Biological Laboratories, Campbell, CA, USA) was intraperitoneally injected at a dose of 400 ng on post-immunization days 0 and 2. A booster injection of MOG35-55 was given on day 7. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice were also injected intraperitoneally with 300 ng Pertussis toxin (PT; List Biological Laboratories) in 200 L PBS (final concentration 1.5 g/mL) on the day of immunization and 2 d later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)