Bacterial Toxin Research Citations

… This was followed by an intraperitoneal (I.P.) injection of 250 ng of Bordetella pertussis toxin (List Biological Laboratories Inc.) in PBS at day 0 and day 2 post-immunization. Donor mice were sacrificed day 10 post-immunization and spleens and lymph nodes were harvested …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice were immunized weekly for 1, 6, or 12 weeks by oral gavage with ground blanched peanut (Western Mixers Produce & Nuts), roasted and unsalted peanut (Whole Foods Market), or wheat flour (Bobs Red Mill Company) or OVA (Sigma) at 5 mg food antigen, with or without 10 µg of CT (List Biologicals, lot nos. 10165A1 and 10167A2) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Table 2
Injected tracers used in this study

Cholera toxin subunit B CTb List Biological Laboratories Product #104 (Spangler 1992) 0.1% solution in dH20

Stereotaxic Injections

Mice were anesthetized with isoflurane (0.5–2.0%) and placed in a stereotactic frame (Kopf 1900 or 940). We made a midline incision and retracted the skin to expose the bregma and the skull over the brain. Through a pulled glass micropipette (20–30–μm inner diameter), we injected Fluorogold (FG), cholera toxin subunit B (CTb), or an AAV (Table 2).

Table 4
Antisera used in this study

Cholera toxin B (CTB) Purified choleragenoid, the B subunit of cholera toxin List Biological, goat polyclonal, cat. #703, lot #7032A9, RRID: AB_10013220 1:10 000

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

B Cell Enrichment and Culture

… Cells were maintained in 5% CO2 at 37°C. B cells were stimulated with LPS 0111:B4 (10 μg/ml, List Biological Laboratories, Campbell, CA, United States), …

• Product #201 – LPS from Escherichia coli O111:B4

Monocyte cell culture and stimulation

… At specified time points, culture supernatants were collected for measurement of TL1A and cells were harvested for qRT-PCR for measurement of TL1A mRNA. LPS (Ultrapure Salmonella Minnesota R595, List Biological Laboratories Inc., Campbell, CA) …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

EAE induction and ATO treatment

…. Pertussis toxin (500 ng; List Biological Laboratories Inc., CA, USA) was administered intraperitoneally on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… A: representative images of LifeAct-Ruby HIEs over time (116 h) after exposure to Fluorobrite DMEM medium alone or purified TcdA (List Biologicals) in Fluorobrite DMEM …

• Product #152C – Toxin A from Clostridium difficile

Depletion and monitoring of platelets

… To model thrombocytopenia by diphtheria toxin receptor-mediated megakaryocyte depletion, PF4-iCre mice were crossed to ROSA26iDTR (iDTR) mice (iDTRPf4). On the first day of depletion, 400 ng of diphtheria toxin (DT) (List Biological Laboratories, Cat. No. 150) was injected i.p. into Cre-positive mice and Cre-negative littermates. On the second and fourth days of depletion, 200 ng of DT was injected i.p. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… To identify the neurochemical nature of LT cells projecting to the LA and the AStr, single retrograde tracings were carried out using CTB (List Biological Laboratories, 104) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Experimental Autoimmune Encephalomyelitis

Experimental autoimmune encephalomyelitis (EAE) was induced as described previously (Radbruch et al., 2016). Briefly, mice were immunized subcutaneously with 250 μg of MOG35–55 (Pepceuticals, United Kingdom) emulsified in CFA (BD Difco, Germany) and received 400 ng pertussis toxin (PTx, List Biological Laboratories, Inc.) intraperitoneally at the time of immunization and 48 h later. Intravital multi-photon microscopy was carried out at the peak of disease.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)