Bacterial Toxin Research Citations

Materials and Methods

1.1 “Main reagents and instruments” Diphtheria toxin (diphtheria toxin, DT, List Biological Laboratories, USA); …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Neutrophil Recruitment Models

Lipopolysaccharide (LPS) challenge: Intratracheal instillation of LPS from E. coli (LIST Biologicals Campbell, CA) into lungs of Zfp30+/+ and Zfp30-/- mice was carried out at a dose of 0.3 mg per kg of body weight using previously described methods (Limjunyawong et al. 2015, Mock et al. 2020). …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

… Pertussis toxin (200 ng; List Biological Laboratories) in PBS was administered ip on days 0 and 2. One group of mice received adjuvant and pertussis toxin only and served as the control. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… To deplete Tregs, diphtheria toxin (DT) (List Biologicals Laboratories, Campbell, CA) was given intraperitoneally to Foxp3DTR or Foxp3EGFP mice before and after LPS …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… The cytotoxic titer was defined as the reciprocal of the highest dilution that produced rounding in 80% of Vero cells for each sample. Vero cells treated with purified C. difficile toxins (A and B) and antitoxin (List Biological Labs, 152C and 155C; TechLabs, T5000) were used as controls …

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

Reagent type (species) or resource

Antibody Goat Anti-Cholera Toxin B Subunit List Biological Laboratories, Inc Catalog #: 703

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Light-Chain Activity High-Performance Liquid Chromatography (LCA-HPLC) Assay

… Next, 25 μL of 200 μM SNAPtide 520 (List Biological Laboratories Inc., Campbell, CA, USA) was added to each reaction tube (equivalent to 13.3 μM SNAPtide 520) followed by incubation at 30 °C for 20 h (digestion step). Reactions were quenched following the addition of 25 μL of 5% trifluoroacetic acid (TFA). The contents of each tube were then transferred to HPLC vials for analysis. The fluorescently-labeled cleavage product(s) were separated and detected via a reverse-phase (RP)-HPLC method [28] using a Waters 2695 XE Separations Module and a Waters 2475 Multi λ Fluorescence Detector (Waters Corp., Milford, MA, USA). In brief, 25 µL of cleavage products were loaded onto a Symmetry C18 column (300 Å, 3.5 µm, 4.6 mm × 150 mm; Waters Corp.), maintained at 35 °C. Separation was accomplished using a 10−90% CH3CN (0.1% TFA) gradient for 30 min, with a flow-rate of 1 mL/min. The column effluent was monitored fluorescently (excitation λ = 322 nm, emission λ = 420 nm) to detect the o-aminobenzoic acid fluorophore on the N-terminal cleaved fragment of SNAPtide 520. SNAPtide cleavage product (#529, List Biological Laboratories, Inc.) was utilized to identify retention time of the cleavage product, as #529 is the unquenched calibration peptide for SNAPtide 520 substrate for C. botulinum type A neurotoxin.

• Product #520 – SNAPtide® Peptide Substrate (o-Abz/Dnp) for C. botulinum Type A Neurotoxin
• Product #529 – Unquenched Calibration Peptide for SNAPtide® 520 Substrate for C. botulinum Type A Neurotoxin

… In addition, 150 ng of Pertussis toxin (List Biologic Laboratories, Meudon, France) was given intravenously on the same day and 2 days later …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (500 ng; List Biological Laboratories Inc., Campbell, CA, USA) was intraperitoneally injected on the same day (day 0) and 2 days post-immunization (PI). After immunization, mice were monitored daily for weight loss and clinical signs …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents

… C. difficile TcdA and TcdB toxoids (formaldehyde inactivated; List Biological Laboratories Inc. Campbell, USA) were used at 10µg/mL and 5µg/mL respectively. …