Bacterial Toxin Research Citations

Materials
… LPS derived from Salmonella Minnesota R595 (Re) was purchased from List Biological Laboratories, Inc. (Campbell, CA) and used for proB and proCE activation assays. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… AT-EAE was induced as previously described [6]. In brief, 2D2/TCRMOG mice were immunized with 200 µg of MOG3555 (MEVGWYRSPFSRVVHLYRNGK; Genscript, RP10245) emulsified in CFA (BD Difco, 263810) and treated with 200 ng of pertussis toxin (List Biological Laboratories, 179B) i.p. on the day of immunization …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Chemicals, Peptides, and Recombinant Proteins

Protective antigen (PA), recombinant from Bacillus anthracis List Biological Laboratories 171C

Stimulation assays

… For the stimulation of the NLRC4 inflammasome, human macrophages were primed with 200 ng ml-1 LPS for 3 hours before 2 μg ml-1 LFn-PrgI and 0.5 μg ml-1 PA (from Bacillus anthracis) were added to the culture medium for 2 hours (Zhao et al., 2011).

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

EAE Induction and Clinical Evaluation

EAE was induced as previously described [7,8,9]. Briefly, mice were immunized by the subcutaneous injection of 200 μL of a mixture of recombinant myelin oligodendrocyte glycoprotein 35-55 (MOG35-55, AnaSpec, Fremont, CA, USA) in a mixture of incomplete Freund’s adjuvant (IFA, Sigma-Aldrich, St. Louis, MO, USA) and Mycobacterium tuberculosis H37RaA (Difco Laboratories, Detroit, MI, USA). Pertussis toxin (PT, List Biological Laboratories, Campbell, CA, USA) was intraperitoneally injected at a dose of 400 ng on post-immunization days 0 and 2. A booster injection of MOG35-55 was given on day 7. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice were also injected intraperitoneally with 300 ng Pertussis toxin (PT; List Biological Laboratories) in 200 L PBS (final concentration 1.5 g/mL) on the day of immunization and 2 d later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… This was followed by an intraperitoneal (I.P.) injection of 250 ng of Bordetella pertussis toxin (List Biological Laboratories Inc.) in PBS at day 0 and day 2 post-immunization. Donor mice were sacrificed day 10 post-immunization and spleens and lymph nodes were harvested …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice were immunized weekly for 1, 6, or 12 weeks by oral gavage with ground blanched peanut (Western Mixers Produce & Nuts), roasted and unsalted peanut (Whole Foods Market), or wheat flour (Bobs Red Mill Company) or OVA (Sigma) at 5 mg food antigen, with or without 10 µg of CT (List Biologicals, lot nos. 10165A1 and 10167A2) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Table 2
Injected tracers used in this study

Cholera toxin subunit B CTb List Biological Laboratories Product #104 (Spangler 1992) 0.1% solution in dH20

Stereotaxic Injections

Mice were anesthetized with isoflurane (0.5–2.0%) and placed in a stereotactic frame (Kopf 1900 or 940). We made a midline incision and retracted the skin to expose the bregma and the skull over the brain. Through a pulled glass micropipette (20–30–μm inner diameter), we injected Fluorogold (FG), cholera toxin subunit B (CTb), or an AAV (Table 2).

Table 4
Antisera used in this study

Cholera toxin B (CTB) Purified choleragenoid, the B subunit of cholera toxin List Biological, goat polyclonal, cat. #703, lot #7032A9, RRID: AB_10013220 1:10 000

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

B Cell Enrichment and Culture

… Cells were maintained in 5% CO2 at 37°C. B cells were stimulated with LPS 0111:B4 (10 μg/ml, List Biological Laboratories, Campbell, CA, United States), …

• Product #201 – LPS from Escherichia coli O111:B4

Monocyte cell culture and stimulation

… At specified time points, culture supernatants were collected for measurement of TL1A and cells were harvested for qRT-PCR for measurement of TL1A mRNA. LPS (Ultrapure Salmonella Minnesota R595, List Biological Laboratories Inc., Campbell, CA) …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)