Bacterial Toxin Research Citations

Muscle injury model

… Eight-week-old BALB/c mice were immunized three times each, at 1-week intervals, with 200 µL emulsion containing 1 mg myosin and 100 µg complete Freund’s adjuvant (Chondrex Inc., Washington, USA). This emulsion was injected bilaterally into the hock (first immunization), tail base (second immunization), and flanks (third immunization). Pertussis toxin (List Biological Laboratories Inc., California, USA) was injected intraperitoneally (500 ng in 100 µL saline) 1 h after the first immunization. We confirmed the elevation of serum antibody titers against myosin by enzyme-linked immunosorbent assay and by immunohistochemical staining of CD8+ T cells and major histocompatibility complex class I (Supplementary Fig. 1A, B).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Chemicals, Peptides, and Recombinant Proteins

Pertussis toxin List Biological Laboratories Cat# 181

EAE induction

Naive CD4 T cells from 2D2 mice were differentiated under Th17 conditions in the presence of vehicle or 2.5 ng/ml oligomycin for 5 days, and 1×106 CD4 T cells were transferred into age- and sex- matched Rag1−/− mice. Recipient mice were injected with 200 ng of pertussis toxin (List Biological Laboratories) intraperitoneally on day 0 and day 2.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE

… In case of the semi-therapeutic model, EAE was induced by subcutaneous injection of 100 µg of MOG35-55 antigen (GenScript, Nanjing, China) in complete Freund’s adjuvant emulsion (Chondrex, Redmond, WA, USA). At day 0 and day 2 after immunization, 200 ng of pertussis toxin (List Biological Laboratories Inc., Campbell, CA, USA) was injected intraperitoneally. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animals and Regents

… Pertussis toxin (PTX) was purchased from List Biological Lab …

Induction and assessment of EAE

EAE was induced as previously described [19]. Mice received a subcutaneous injection in the flank of 200 μg MOG35–55 peptide emulsified in CFA containing 500 μg Mycobacterium tuberculosis H37RA. Immediately thereafter and again 48 h later, the mice were given an intraperitoneal injection of 300 ng PTX in 100 μl phosphate-buffered saline (PBS). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunization

Eight micrograms of OVA (Sigma) and 2 μg of cholera toxin subunit B (List Biological) in 6 μL of sterile PBS or 6 μL of PBS were administered intranasally. Mice were sacrificed 7 days later and parotid glands were removed and frozen. For detection of CD3+ cells, representative sections were prepared for fluorescent immunohistochemistry. The number of CD3+ cells was counted in 15 high power fields (hpf).

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… After 72 h of puromycin selection, cells were collected and divided into two groups (2.8×107 cells/group) and cultured for 2 weeks in either the presence (resistant) or absence (control) of 2 ng/ml Shiga toxin (List Biological Laboratories) …

Author did not specify which List Labs Shiga Toxin was utilized for their research.
List Labs offers the follows:

https://listlabs.com/product-information/shiga-toxins/

… B. anthracis lethal factor (LF) was acquired from List Biologicals. …

• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis

EAE induction

To induce EAE disease, complete Freund’s adjuvant, CFA (#F5881, Sigma) containing 400 μg Mycobacterium tuberculosis, Mtb (#DF3114-33-8, Fisher), and 100 μg myelin oligodendrocyte glycoprotein 35–55 peptide (MOG35–55, United Peptides) were subcutaneously administered at 0 and 7 days post-induction (dpi). Pertussis toxin (200 ng/mouse) (#181, List Biological Laboratories, Inc.) was administered on days 0, 2, and 7 dpi. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Pertussis toxin List Biological Laboratories Cat # LB181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Reagents

… Lipopolysaccharide (E. coli, serotype O111:B4 L2630) was from List Biological Laboratories (Campbell, CA) …

Alcohol Exposure and LPS Challenge

Mice were exposed to ethanol (2 or 4 g/kg), propanol (2 g/kg), isopropanol (2 g/kg) or PBS via intraperitoneal injection. One hour later, a lethal dose of LPS (10 mg/kg, i.p.) was injected. Animals were monitored every 2 h for 36 h post LPS injection.

• Product #201 – LPS from Escherichia coli O111:B4