Bacterial Toxin Research Citations

… Immunofluorescence 1×105 SCC-4 cells/well (biological triplicates) control and Clostridium difficile Toxin A – ToxA (List Biological Labs, Campbell, CA, USA) treated: 1, 2 and 4 µg/mL cultured on three-dimensional MatrigelTM for 24 h. …

• Product #152C – Toxin A from Clostridium difficile

Botulinum neurotoxin and its administration

Research-grade, purified, native BoNT serotype A1 (BoNT-A; 150 kDa) was purchased from List Biological Laboratories (Campbell, CA, USA). It was received as a lyophilized powder, which was reconstituted in 1 mg/mL phosphate buffer saline/bovine serum albumin (0.1%) to obtain a stock solution (0.1 mg/mL), which was aliquoted and kept frozen at −80 °C. Dilutions of aliquots were performed with 0.2% GPB to obtain the final concentrations (see below). …

… Adjuvanted formulations included 2µg of cholera toxin (List Biological Laboratories, 101B), 10µg of CTB (List Biological Laboratories, 103B), or …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Ultrapure S. minnesota R595 LPS (List Biologicals) at a concentration of 1 g/ml was used for all cell culture LPS treatments unless otherwise noted. …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

… LPS from Salmonella minnesota R595 (Re) List Biological Laboratories, Inc. 304 …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Reagents

Lipopolysaccharide (LPS), macrophage-activating lipopeptide of 2 kDa (MALP-2), and polyinosinic:polycytidylic acid (poly[I:C]) were purchased from List Biological Lab (Campbell, CA, USA), Alexis Biochemical (San Diego, CA, USA), and Amersham Biosciences. …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

Experimental Autoimmune Encephalomyelitis (EAE)

For induced EAE, mice were immunized subcutaneously with 100 g of myelin oligodendrocyte glycoprotein (MOG)35-55 peptide emulsified in complete freund adjuvant (CFA), which was made from IFA(Difco) plus mycobacterium tuberculosis (Difco). Mice were i.p. injected with 200 ng of pertussis toxin (List Biological,#181) on the day of immunization and 2 days later. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE induction and evaluation

Unless otherwise noted, EAE was induced as follows: MOG35-55 peptide (100 µg in 100 µl PBS) emulsified in CFA (100 µl including 2 mg/ml hkMtb) was subcutaneously (s.c.) injected into lower flanks of mice. Intraperitoneal injection of pertussis toxin (List Biologicals; 200 ng in 200 µl PBS) was performed on day 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Active experimental autoimmune encephalomyelitis (aEAE) induction.

… Briefly, an emulsion of MOG35-55 and CFA was injected subcutaneously in mouse flanks and at the tail base at day 0; in addition, 300 ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA) was injected intraperitoneally at day 0 and day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE immunizations and scoring

… On days 0 and 2, mice received an intraperitoneal injection of 250 ng pertussis toxin (List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)