Bacterial Toxin Research Citations

Viral construct and anatomical tracer injections, iontophoresis, and cannula implants

… For delivery of retrograde anatomical tracers to the PB of rats and mice, we used either cholera toxin subunit B (CTB, List Biological Labs) …

Retrograde immunohistochemical identification of CeA-PB neurons

We injected PB of GAD-GFP mice and Sprague-Dawley rats with CTB (see above).
Between 7 to 14 days post injection, we perfused, collected, and sectioned tissue.
We processed free-floating sections (50 µm) for double-label immunohistochemistry with antibodies against CTB (goat anti-CTB; List Biological Labs, Inc.; product #703; 1:20,000) and …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE induction and clinical evaluation

EAE was induced in 8-week-old female C57BL/6 mice by subcutaneous administration of 250 μg MOG35–55 peptide (synthesized by Sigma Laboratories, Israel) into the left paralumbar region. The MOG35–55 peptide had been emulsified in complete Freund’s adjuvant (CFA) containing 5 mg/ml heat-killed Mycobacterium tuberculosis. Immediately after this injection and at 48 h after the injection, the mice were intraperitoneally administered 200 ng of the pertussis toxin (List Biological Industries, San Diego CA, USA). All the animals were examined daily and evaluated for clinical signs of disease. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (500 ng; List Biological Laboratories, Inc., Campbell, CA, USA) was intraperitoneally injected into the rats at days 0 and 2 PI. Rats were observed daily post-immunization for clinical signs of EAE. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tissues and cells

… MDA-MB-246, MDA-MB-231, T47D and ZR-75-30 cells were used as model BCa lines (Chinese Cell Bank). Cells were cultured in DMEM plus 10% FBS and 1% (v/v) pen/strep at 37 °C in a 5% CO2 humidified atmosphere. The normal epithelial breast cell line MCF-10A was cultured in MEBM basal medium (Lonza, Basel, Switzerland) supplemented with the MEGM Single Quot Kit (Lonza) and cholera toxin (List Biological Labs, Campbell, CA).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Female 6 week-old BALB/c mice (purchased from Charles River Laboratories, Saint Germain sur l`Arbresle, France) were administered by intragastric gavage: PBS (0.2 mL), cholera toxin (10µg; CT, List Biologicals, Campbell, USA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

SCFAs modulation of T-dependent and T-independent antibody responses

To analyze local (intestinal) and systemic T-dependent antibody response to OVA, C57BL/6 mice fed a fiber or no-fiber diet, and SCFAs water or plain water ad libitum, were administered OVA (20 mg) plus mucosal adjuvant cholera toxin (List Biological Labs, 20 μg) in 500 μl PBS via intragastric gavage once a week for 4 weeks, and killed 1 week after the last OVA and cholera toxin administration for all studies. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… CHAPTER 2: METHODS 2.1 Reagents Lipopolysaccharide (Salmonella Minnesota R595) was purchased from List Biological Laboratories, Campbell, CA, USA. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

• Product #149A – Diphtheria Toxin CRM197, Mutant

Briefly, the sections were rinsed in a solution of Tris buffered saline (TBS-T, 0.05M, pH 8.6) with Triton ×100 at 0.5% before overnight incubation at 4°C with primary antibody against CB (Goat anti-choleragenoid; List Biological Laboratories-Product numero: 703; 1:10?000)

4.2.9 Animal model

4.2.9.1 EAE

EAE was induced as described before (Zinser et al., 2004). In brief, on day 0, 10-12 week old female C57BL/6N mice were subcutaneously immunized with a 1:1 ratio of MOG peptide35-55 (50 µg; Charité, Berlin, Germany) and complete Freund`s adjuvant (CFA; Sigma-Aldrich) enriched with 10 µg/ml Mycobacterium tuberculosis (H37Ra; Difco/BD Biosciences). Furthermore, pertussis toxin (200 ng; List Biological Laboratories, Campbell, California, USA) was injected intraperitoneally on days 0 and 2.