Bacterial Toxin Research Citations

2.2. Antigens

Vibrio cholerae toxin (CT; lot 10154B5A) and diphtheria toxoid (DPT; lot 1517B3) were from List Biological Labs (Campbell, CA, USA); …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
• Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae

… These inoculations were followed with pertussis toxin (PTx) (List Biologicals) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Next, the cells were stimulated by adding 100 ng/ml LPS (ultrapure smooth LPS from E. coli O111:B4; List Biological Laboratories, Campbell, CA) for 1 h or 4 h. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Pertussis toxin (200 ng, List Biological Laboratories) in PBS was administered i.v. on days 0 and 2. Mice were examined daily and scored for disease severity using the standard scale: 0, no clinical signs; 1, limp tail; 2, paraparesis (weakness, incomplete paralysis of one or two hind limbs); 3, paraplegia (complete paralysis of two hind limbs); 4, paraplegia with forelimb weakness or paralysis; 5, moribund or death. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 1:1,000 for antiEF and antiLF sera, and antiPA antibody (List Labs, Campbell, California). …

First step c-Fos/CTb

Of the 28 animals used, 8 showed a suboptimal localization of the CTb and were excluded from this part of the study, therefore number of animal for each group changed to: Torpor n=4, Fasting n=4, Cold Exposure n=4, Control n=8. Following the blocking solution, all slices were incubated at room temperature overnight with the following primary antibodies: i) goat Anti-CTb (List Biological Laboratories); …

Second step neuronal typization

… 5.  Slices were then rinsed twice in PBS with 0,3% (v/v) Triton X-100 and incubated overnight at room temperature with: i) goat Anti-CTb (List Biological Laboratories; 1:10000); ii) rabbit Anti-c-Fos (Calbiochem; 1:10000).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

E2 pellet implantation and induction of EAE

… Additionally, mice were administered 75 ng of pertussis toxin (Ptx, List Biologicals, Campbell, CA, USA) via an intraperitoneal (i.p.) injection on the day of immunization and 200 ng i.p. 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The retrograde tracer, cholera toxin subunit (CTB; List Biological Labs, Campbell, CA, USA CTB serum (1:10000, List Biological Labs, Campbell, CA, USA) for 2 days. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Vaccine preparation and immunization of mice

… For I.N. immunizations, WCA (100 μg) was diluted in saline and combined with CT (List Biological Laboratories, Campbell, CA) (0.1 μg/10 μl) and administered to the nares of mice twice at weekly intervals. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Study design and animal model:

… EAM was induced by intraperitoneal injection of pertussis toxin (400ng, List Biological Laboratories) followed by subcutaneous injection of MyHC peptide …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)