Bacterial Toxin Research Citations

Exposures

Mice received ultrapure E. coli O111:B4 LPS (List Biological Labs, Campbell, CA) (50 ml of 1 mg/ml in
PBS), or control PBS vehicle only, by oropharyngeal aspiration and were phenotyped 24 hr later. For
systemic LPS exposure, mice received 10 mg/kg LPS or PBS by intraperitoneal injection and were
phenotyped 24 hr later. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Breakdown of the BBB and the passive transfer of IgG to mice

After completing one session of EEG recording under normal conditions, the mice received IgG by passive transfer. Before IgG transfer, the blood–brain barrier was disrupted using a previously described method [35]. In brief, 50 μg complete Freund’s adjuvant (CFA, Sigma-Aldrich, USA) containing heat-killed H37Ra Mycobacterium tuberculosis (Difco, USA) in 50 μl was subcutaneously injected into each of four sites on the hind flank. In addition, mice received an intraperitoneal (i.p.) injection of 200 ng pertussis toxin (PTx, List Biological Laboratories, USA) in 0.2 ml PBS. The injection of PTx was repeated again 3 days later. On the 7th day after the injection of CFA, animals were injected intravenously with PBS (as vehicle) or test or control IgG (200 μg in 0.2 ml PBS). Electrophysiological recording was conducted again 1, 24, 48, and 72 h after the injection of IgG.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Rat treatment, CSF and blood collection, leukocyte count

Eight-day-old (P8) rats were injected intraperitoneally (i.p.) with 1 or 5 mg/kg P3C (Invivogen, France), 0.3 mg/kg ultrapure LPS (List Biological Laboratory, USA), or 0.9% saline. …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

… emulsified in complete Freund’s adjuvant (CFA; 1.5mg/ml) followed by inoculations with 300ng of pertussis toxin, Bordatella pertussis, (List Biological Labs) on the day of induction and 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… LPS was purchased from List Biological Laboratories, Inc. …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

Injection of a retrograde tracer into the parafacial respiratory group

In five animals, pFRG injections of the retrograde tracer cholera toxin b (CTb, 1% in deionized water; List Biological, Campbell, CA) were unilaterally performed. For pFRG injections, the dorsal surface of the brain was exposed via an occipital craniotomy. The stereotaxic coordinates for targeting the pFRG were 2.7 mm caudal from lambda, 2.6 mm lateral from midline, and 8.7 mm ventral from the skull surface. The CTb (30 nl) was delivered by pressure through glass pipettes. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… CTb Gt Polyclonal List Biological Labs 102946 502 7032A10 1:5000 1:10000 …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

C. difficile in vitro Vero cell cytotoxicity assay

… The cytotoxic titer was defined as the reciprocal of the highest dilution that produced rounding in 80% of Vero cells for each sample. Vero cells treated with purified C. difficile toxins (A and B) and antitoxin (List Biological Labs, 152C and 155C; TechLabs, T5000) were used as controls.

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

Key Resources Table

LPS ultra pure from Salmonella minnesota R595 List Biological Laboratories, INC. Cat# 434

Infections and stimulations

MDDCs were infected or stimulated with innate agonists between day 4 and day 6 after differentiation. For infections with HIV-1 and related lentiviruses, except where noted otherwise, Vpx+ virus-like particles were used to overcome virus restriction (1 mL supernatant for 1 × 10ˆ7 cells) (Johnson et al., 2018, Manel et al., 2010). MDDCs were counted on day 4 and resuspended at 800,000 cell per ml in fresh medium with GM-CSF, IL-4, and polybrene (1 μg/ml) and then reseeded into appropriate culture vessels. For most assays, MDDCs were infected at a density of 800,000 cells per ml by diluting virus in MDDC media (without cytokines or polybrene) to a final volume normalized to controls. Innate and inflammatory stimuli were used at the indicated concentrations: poly(I:C) (InvivoGen, 10 μg/ml); LPS (List Biological Laboratories, INC, 1 ng/ml).

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

… Pertussis toxin (List Biological) (400 ng) was injected on the day of the immunization and again two days later …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)