Bacterial Toxin Research Citations

Antibody selection in microtiter plate

The antibody selection was performed as described previously with modifications33. In brief, for the panning procedure, the antigen was immobilized on a Costar Highbinding 96 well plate (Corning, New York, USA). 1 µg of DT (List Biological Laboratories, Campbell, USA) was diluted in PBS (137 mM NaCl; 1.76 mM KH2PO4 × 2 H2O) and coated in the wells at 4 °C overnight. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Antibody ELISA assay. BPZE1 and BP611-98 bacterial lysates were prepared as described in the Supplemental Materials and Methods. FHA, PT, PRN, FIM2/3, and ACT were purchased from List Biological Laboratories, USA …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #186 – Fimbriae 2/3 from B. pertussis
• Product #187 – Pertactin from B. pertussis (69 kDa Protein)
• Product #188L – Adenylate Cyclase Toxin, Recombinant from B. pertussis

… At the same time and 3 d later, pertussis toxin (300 ng pertussis toxin in PBS; List Biological) was ip injected. On day 21 after MOG35-55 injection, B cells were sorted by B220 MicroBeads. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Analysis of axonal regeneration following SCI.

Adult 8-week-old cre+Pfn1 mice and 15-week-old female C57BL/6 mice were deeply anesthetized with isoflurane. Laminectomy was performed at the thoracic T8 to T9 level and the spinal cord was cut using a micro feather ophthalmic scalpel (Feather, Safety Razor Co). For CL experiments, animals were subjected to a sciatic nerve transection 1 week prior to SCI. In cre+Pfn1 mice, analysis of dorsal column axon regeneration after either SCI or CL was performed 4 weeks after injury. Dorsal column axons were traced by injecting 2 μL of 1% CT-B (List Biologicals, 103B) with a 10-μL syringe (Hamilton) into the left sciatic nerve 4 days prior to euthanasia (day 24 after injury). On day 28, mice were perfused with 4% PFA and the spinal cords were postfixed for 1 week at 4°C and later cryoprotected in 30% sucrose. Serial tissue sagittal cryosections (50 μm thickness) were collected for free-floating immunohistochemistry. Sections were incubated overnight at 4°C with goat anti–CT-B primary antibody (1:30,000; List Biologicals, 703).

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… A total of 31.7 nM PA alone (List Labs, no. 171E) or in the presence of 13.6 nM LFN, LFN-ACD, or LFN-RID was added to the media, and cells were incubated for 20 hours at 37C in the presence of 5% CO2 …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Pertussis toxin (200 ng; List Biological Laboratories) was injected intravenously at days 0 and 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Viral construct and anatomical tracer injections, iontophoresis, and cannula implants

… For delivery of retrograde anatomical tracers to the PB of rats and mice, we used either cholera toxin subunit B (CTB, List Biological Labs) …

Retrograde immunohistochemical identification of CeA-PB neurons

We injected PB of GAD-GFP mice and Sprague-Dawley rats with CTB (see above).
Between 7 to 14 days post injection, we perfused, collected, and sectioned tissue.
We processed free-floating sections (50 µm) for double-label immunohistochemistry with antibodies against CTB (goat anti-CTB; List Biological Labs, Inc.; product #703; 1:20,000) and …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE induction and clinical evaluation

EAE was induced in 8-week-old female C57BL/6 mice by subcutaneous administration of 250 μg MOG35–55 peptide (synthesized by Sigma Laboratories, Israel) into the left paralumbar region. The MOG35–55 peptide had been emulsified in complete Freund’s adjuvant (CFA) containing 5 mg/ml heat-killed Mycobacterium tuberculosis. Immediately after this injection and at 48 h after the injection, the mice were intraperitoneally administered 200 ng of the pertussis toxin (List Biological Industries, San Diego CA, USA). All the animals were examined daily and evaluated for clinical signs of disease. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (500 ng; List Biological Laboratories, Inc., Campbell, CA, USA) was intraperitoneally injected into the rats at days 0 and 2 PI. Rats were observed daily post-immunization for clinical signs of EAE. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tissues and cells

… MDA-MB-246, MDA-MB-231, T47D and ZR-75-30 cells were used as model BCa lines (Chinese Cell Bank). Cells were cultured in DMEM plus 10% FBS and 1% (v/v) pen/strep at 37 °C in a 5% CO2 humidified atmosphere. The normal epithelial breast cell line MCF-10A was cultured in MEBM basal medium (Lonza, Basel, Switzerland) supplemented with the MEGM Single Quot Kit (Lonza) and cholera toxin (List Biological Labs, Campbell, CA).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae