Bacterial Toxin Research Citations

… Female 6 week-old BALB/c mice (purchased from Charles River Laboratories, Saint Germain sur l`Arbresle, France) were administered by intragastric gavage: PBS (0.2 mL), cholera toxin (10µg; CT, List Biologicals, Campbell, USA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

SCFAs modulation of T-dependent and T-independent antibody responses

To analyze local (intestinal) and systemic T-dependent antibody response to OVA, C57BL/6 mice fed a fiber or no-fiber diet, and SCFAs water or plain water ad libitum, were administered OVA (20 mg) plus mucosal adjuvant cholera toxin (List Biological Labs, 20 μg) in 500 μl PBS via intragastric gavage once a week for 4 weeks, and killed 1 week after the last OVA and cholera toxin administration for all studies. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… CHAPTER 2: METHODS 2.1 Reagents Lipopolysaccharide (Salmonella Minnesota R595) was purchased from List Biological Laboratories, Campbell, CA, USA. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

• Product #149A – Diphtheria Toxin CRM197, Mutant

Briefly, the sections were rinsed in a solution of Tris buffered saline (TBS-T, 0.05M, pH 8.6) with Triton ×100 at 0.5% before overnight incubation at 4°C with primary antibody against CB (Goat anti-choleragenoid; List Biological Laboratories-Product numero: 703; 1:10?000)

4.2.9 Animal model

4.2.9.1 EAE

EAE was induced as described before (Zinser et al., 2004). In brief, on day 0, 10-12 week old female C57BL/6N mice were subcutaneously immunized with a 1:1 ratio of MOG peptide35-55 (50 µg; Charité, Berlin, Germany) and complete Freund`s adjuvant (CFA; Sigma-Aldrich) enriched with 10 µg/ml Mycobacterium tuberculosis (H37Ra; Difco/BD Biosciences). Furthermore, pertussis toxin (200 ng; List Biological Laboratories, Campbell, California, USA) was injected intraperitoneally on days 0 and 2.

Sensitization of mice.

Mice received 1 mg of cashew protein extract supplemented with 10 μg of Cholera Toxin (List Biological Laboratories) per intragastric route, at a rate of one intragastric administration per week for 6 consecutive weeks.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… LPS (E. coli O11: B4-derived) was purchased from List Biologicals (Campbell, CA, USA), …

• Product #201 – LPS from Escherichia coli O111:B4

… Mice also received two intraperitoneal injections of 350 ng of pertussis toxin (List biological laboratories, Inc) at the time of induction and 48 hours later …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

3.2. EAE induction

… After MOG35-55 injection, mice received varying amounts (as listed) of Bordetella pertussis toxin (PTX) in glycerol buffer depending upon the experiment,
intraperitoneally (List Biological Laboratories, Campbell, CA; provided with Hooke Kit™ for EAE Induction) on day 0 and on day 1. …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)