Bacterial Toxin Research Citations

EAE:

… Briefly, the mice were subcutaneously injected with 200 µg MOG 35-55 in complete Freund’s adjuvant (5 mg/ml, Sigma-Aldrich). Pertussis Toxin (200 ng, List Biological Laboratories) was injected intravenously twice on 0 and 2 days postimmunization (dpi.) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Instead of BDA cholera toxin B subunit (0.5% in 0.1 N PB; CTB, low-salt cholera toxin B subunit, List Biological Laboratories, Campbell, CA) was injected iontophoretically by passing positive current (4 A, 1 Hz, 10 % duty cycle) for 10 minutes into the GL of the medial …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Antibodies and chemicals:

… pertussis toxin (179B) was from List Labs. …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Where applicable, macrophages were activated by treatment with 100 U/ml interferon- (PeproTech, NJ) and 10 ng/ml lipopolysaccharide (List Biological Laboratories, CA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… RGCs were labeled with 1% cholera toxin B subunit (CTB; List Biological Laboratories, Campbell, CA) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… The retrograde tracers CTB (0.5% diluted in distilled water; List Biological) or fluorogold (FG; 2% in 0.1M cacodylate buffer; Fluorochrome) were iontophoretically injected (for CTB, 7/7s on/off duty cycle, 1A; for FG, 2/2s on/off duty cycle, 1 …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Vero cell cytotoxicity assay of C. difficile positive fecal samples

… Control containing purified C. difficile toxins (A and B; List Biological Labs, CA, USA) and antitoxin were included. …

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile
• Product #753A – Anti-Clostridium difficile Toxin A (Chicken lgY)
• Product #754A – Anti-Clostridium difficile Toxin B (Chicken lgY)

IL-12p40 flow cytometry assays:

For determination of IL-12p40 production by activated DCs andmacrophages, GM-CSF cultures were set up from get40 mice as de-scribed in section 2.2.1. On day 9, cells were stored at 4 C for 30 minand afterwards scraped off the dishes. Cells were centrifuged, re-suspended in fresh medium and counted for culturing in non-treated12-well plates (Sigma-Aldrich). Cells were seeded at a density of5 105cells/well for a final volume of 1 ml and initially treated with 5concentrations of 01 M CpG 2216 (TIB Molbiol) or 010 ng/ml lipopolysaccharide (LPS; List Biological Laboratories, Inc.), respectively,for 16 h to determine suboptimal stimulation conditions. For testing ofnatural compounds, cells were incubated with 0.1, 1 or 10 M of nat-ural products with or without 0.1 M CpG 2216 or 1 ng/ml LPS or leftuntreated at 37 C for 16 h. Untreated cells or cells treated with 0.1 MCpG 2216 or 1 ng/ml LPS were used as controls. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… Endotoxins from Escherichia coli O55:B5, E. coli O111:B4, Salmonella minnesota R595 Re, S. typhimurium were purchased from List Biological Laboratory, Inc. (CA, USA). …

• Product #203A – LPS from Escherichia coli O55:B5
• Product #304 – LPS from Salmonella minnesota R595 (Re)
• Product #225 – LPS from Salmonella typhimurium

Stereotactic surgery (6-OHDA infusion and BoNT-A Injection):

… 6-OHDA infused and control rats then received BoNT-A injection (List Biological Laboratories, Campbell, California, USA) at the right EPN four weeks after 6-OHDA surgery. Delivery of 0.5 ng of BoNT-A into the EPN was performed by injection of 0.5 uL of BoNT-A solution (0.5 L of 1 ng/uL BoNT-A dissolved in phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)). Infusions were performed over 5 minutes via a 31G injection cannula attached to a 10 uL Hamilton syringe. Correspondingly, vehicle animal groups were administered with an equivalent volume of PBS with 0.1% BSA. The stereotactic coordinates with reference to bregma were: AP -2.5 mm, L 3 mm, DV 7.7 mm (Paxinos and Watson, 2009).