Bacterial Toxin Research Citations

Immunization and EAE induction

EAE was induced in WT or Nlrx1^/ female mice as previously described [50]. A mixture of MOG₃₅₅₅ (Genemed Synthesis, San Antonio, TX), CFA (Sigma-Aldrich, St. Louis, MO), and Mycobacterium tuberculosis H37 RA (Difco Laboratories, Detroit, MI) was injected subcutaneously into mice, with each mouse receiving a total of 200 µg MOG₃₅₅₅ and 500 µg Mycobacterium. PTX (List Biological Laboratories, Campbell, CA) (200 ng) was injected intraperitoneally on days 0 and 2. Mice were euthanized after 3 weeks of immunization, and the tissues were collected.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Staphylococcal enterotoxin B (SEB, 0.05 g/ml, Sigma-Aldrich or List Biological laboratories Inc, Campbell, CA), …

… Peanut model: Mice were immunized weekly by oral gavage with 5 mg of ground raw blanched peanut (Western Mixers Produce & Nuts) with or without 10 µg of cholera toxin (List Biologicals) in 200 µL of 0.2 M sodium bicarbonate buffer per mouse. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Pertussis toxin (180) was from List Biological Laboratories. …

Induction and assessment of EAE:

To induce EAE, MOG_35-55 peptide (200 g per mouse) was emulsified with CFA (50 l per mouse, including 4 mg/ml M. tuberculosis H37Ra) and 50 µl incomplete Freunds adjuvant. Pertussis toxin (250 ng per mouse) was applied intravenously on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

… Ultrapure LPS was purified from Salmonella minnesota R595 (List Biological Laboratories) …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

… the half corneal button securely attached to the bottom of the plate, and 1.5 mL of DMEM (Life technology) with 10% serum containing 40 g/mL gentamicin (Life technologies, NY), 1% ITS (Fisher Scientific, PA), and 100 ng/mL cholera toxin (LIST Biological Laboratories) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Infection and stimulation of monocytes:

… Cells were stimulated with 100 ng/ml ultrapure E. coli LPS (List Biological Laboratories, Campbell, CA) and 5 mM ATP (Sigma-Aldrich, St. Louis, MO) for the last 30 min of culture, as indicated. Mock treatment was the addition of the equivalent volume of media (without parasites or LPS) to cells. At the indicated time point, monocytes were pelleted by centrifugation at 500 x g for 5 min. Collected cells were stained, fixed, or lysed accordingly, as described below.

Author did not specify which ULTRA PURE E. coli LPS was utilized; List Labs provides the following:

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4
• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

… Cholera toxin (CT) List Biological Laboratories Cat#100B …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Ultrapure LPS (Escherichia coli 111B4) List Biological Laboratories. INC Cat# 421…

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Reagents:

Purified LPS from Escherichia coli was obtained from List Biological Laboratory Inc. (Campbell, CA, USA) and dissolved in endotoxin-free water. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/