Bacterial Toxin Research Citations

THP-1 differentiation and stimulation.:

… The differentiated THP-1 cells were stimulated with the addition of 1.510⁷ CFU/well of live or heat-inactivated bacteria added in isolation or in combination with 1µg/ml purified CT or CTB (both from List Biologicals, Campbell, CA, USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… CT (10 µg/ml, List Biological Laboratories) was dissolved alone or in combination with CpG-ODN and curdlan in PBS, emulsified with IFA, and then injected intramuscularly as described above. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Experimental Autoimmune Myocarditis:

… For this study, mice were backcrossed onto the BALB/c background as it is permissive for myocarditis [51]. In addition, the mice were injected intraperitoneally with 500 ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA) dissolved in PBS. The mice were monitored for at least one week following the injections. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free) List Biological Laboratories Cat# 181…

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Immunohistochemistry:

… anti-CTB at 1:10,000 (List Biological Laboratories, Campbell, CA). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… CTb, Goat, 703, 1:1000, List Biological. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… 50µl of the emulsion were injected in each axillary fossa. 200ng of pertussis toxin (LIST BIOLOGICAL LABORATORIES, INC) were administered intravenously at the time of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell culture:

MCF-10A cells were obtained from ATCC (Manassas, VA, USA) and were grown using Dulbeccos Modified Eagles Medium/Nutrient Mixture F-12 (DMEM/F12, 1:1; Invitrogen, Carlsbad, CA, USA) supplemented with 5% horse serum (Invitrogen), 20ng/ml recombinant human epidermal growth factor (EGF; R&D systems, Minneapolis, MN, USA), 0.5g/ml hydrocortisone (Sigma, St. Louis, MO, USA), 100ng/ml cholera toxin (List Biological Lab, St, Campbell, CA, USA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

– List Biological Laboratories Inc., Campbell, CA, USA; Cat# 703; goat, polyclonal; RRID: AB_10013220 1:80,000 Anti-PHA-L PHA-L (Phaseolus vulgaris leucoagglutinin) Choleragenoid, List Biological Laboratories Inc., Campbell, CA, USA) in distilled water …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Author did not specify which CTB was utilized.  List Labs has provided Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae) and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).  The former has been discontinued.  The latter may be substituted for the former, if the researcher takes into account the difference in the buffer in which each product is provided and the amount of protein per vial.  When Product #103B was reconstituted with 200 l of water, it contained 1 mg of protein in 0.05 M Tris, 0.2 M NaCl, 0.001 M Na₂EDTA, 0.003 M NaN_3, pH 7.5.  When Product #104 is reconstituted with 0.25 ml water, it contains 0.5 mg of Cholera Toxin B Subunit (choleragenoid) in 0.01 M Sodium Phosphate at pH 7.5.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction and scoring of EAE:

<... Each animal also received 200ng pertussis toxin (List Biological, Campbell, CA, USA) in 0.1ml PBS by intraperitoneal injection at 0h and 48h post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)