Bacterial Toxin Research Citations

Drugs:

… PTX was obtained from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Goat polyclonal anti-Cholera Toxin, B-Subunit List Biologicals RRID: AB_10013220; Cat#703 …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE induction and Evaluation:

… Two hours following immunization, mice were given 200ng pertussis toxin (List Biologicals, Campbell, CA) via intraperitoneal injection, while 400ng was given i.p. on day 2. EAE was induced in 810-week-old female SJL/J mice similarly; however, myelin proteolipid protein peptide (PLP₁₃₉₁₅₁) was used to immunize these mice and 4mg/mL Mycobacterium tuberculosiswas added to CFA. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… After that, the mice received an intraperitoneal injection of 300 ng pertussis toxins (List Biological Lab, USA) on days 0 and 2 post immunization. Then, EAE mice were divided …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

HEK293 cell transfection and stimulation:

… For TLR4/TLR5 co-transfections, HEK293 cells stably transfected with MD-2, CD14, and TLR4 were obtained from Invivogen (Catalog # 293-htlr4md2cd14). Cells were cultured in a 96-well flat-bottomed tissue culture plate at 50 10³ cells/well in cell growth media (DMEM, 4.5 g/l glucose, 50 U/ml penicillin, 50 g/ml streptomycin, 100 g/ml Normocin, 10 g/ml blasticidin, 50 g/ml Hygromycin B Gold, plus 10% fetal bovine serum (FBS) and 1% L-glutamine). The following day cells were transiently transfected using PolyFect transfection reagent (Qiagen, Valencia, CA) and the following DNA: NF-B-ELAM firefly luciferase 50 ng/well, HSV thymidine kinase-Renilla luciferase (pRL-TK, Promega) 5 ng/well, and huTLR5 (cloned into pEF6) and empty pEF6 vector at 110 ng/well as indicated. The transfection complexes were allowed to incubate with the cells for 24 hours. The following morning, the media in the wells was aspirated and the cells were then stimulated with ultrapure Escherichia coli O111:B4 LPS (List Biologicals, Campbell, CA) or ultrapure S. Typhimurium flagellin (Invivogen) suspended in cell growth media. After four hours, cells were lysed with passive lysis buffer and NF-B activation was determined as above.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

… Microglial cells (3 10 4 ) were plated onto glass coverslips and treated with LPS (2.5 g/ml, List Biological Laboratories, #304) and IFN- (25 Units/ml, Sigma, #I4777) to stimulate and activate the cells (Klegeris and McGeer, 2001). …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… and PRN at 0.1 g (PT, cat. 180; FHA cat. 170; and PRN cat. 187; List Biological Laboratories)were determined by ELISA, as described by Quinello et al. [19], with some modifications. …

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

Product #170, Filamentous Hemagglutinin (FHA) from B. pertussis, has been discontinued.  Large quantities may be obtained through Bulk Requests and Customer Orders.

EAE induction, Treg administration, and CNS mononuclear cell isolation:

For EAE induction, 2-month-old Foxp3^Cre or Foxp1^f/fFoxp3^Cre mice were immunized subcutaneously with 200 µg of MOG3555 peptide (GL Biochem, Shanghai, China) emulsified in Complete Freunds Adjuvant (CFA; Sigma). Additionally, mice were injected intravenously with 200 ng of PT (List biological laboratories, Campbell, CA) on d0 and d2 following immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice also received 100ng pertussis toxin (List Biological Laboratories) intraperitoneally on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… The mice were intraperitoneally (ip) injected with 300 ng of pertussis toxin (List Biological Laboratories Inc., USA) at the same time of immunization and 48 h after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)