Bacterial Toxin Research Citations

Subjects:

Two male macaque monkeys (1 Macaca mulatta (Y), 1 Macaca nemestrina (S)) were used for these experiments. Animal Y was 4 years old and weighed 8.2 kg and Animal S was 6 years old and weighed 17.4 kg at the time of surgery. The subjects used in this study were anatomically typical, with no observable abnormalities. MRI-guided injections of anatomical tracers were performed stereotaxically under sterile conditions. Injection sites, tracers used, and volumes of injection for each case are shown in Table 2.1. The animals received bilateral injections of anterograde tracer (fluoroemerald; FE) in superior colliculus and retrograde tracer (choleratoxin B; CTB) in the lateral nucleus of the amygdala; this yielded 4 hemispheres for anatomical analysis.

Table 2.2

Choleratoxin B List Labs

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… lipopolysaccharide (LPS; Escherichia coli-derived, serotype O111:B4; List Biological Laboratories, Campbell, CA); …

• Product #201 – LPS from Escherichia coli O111:B4

… and S1P 1 LysM KO mice immunized with an emulsion containing 200 g Complete Freund’s Adjuvant (CFA), 100 g myelin oligodendrocyte glycoprotein (MOG) peptide 35-55, and 200 ng Bordetella pertussis toxin (lot# 181236A1, List Biological Laboratories …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Clostridium difficile toxin B (TcdB) (List Biologicals ): Spin down toxin tube for 30-45 s at 20,000 g. Reconstitute 20 g of TcdB into 100 L of sterile double-distilled water to obtain a 200 g/mL stock solution. Store at 2-8 C (see Note 5 ). …

• Product #155 – Toxin B from Clostridium difficile

… The sections were blocked in 10% NHS in PBS (30min) then incubated overnight in either mouse anti-TH, (1:20000, ImmunoStar, Hudson, WI, USA, antibody registry: AB_572268) or goat anti-CTB (1:20000, List Biological Laboratories Inc., antibody registry: AB_10013220) in 2% NHS in PBS at RT. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

–Toxin from Clostridium septicum List Biological Laboratories, USA

EAE: 

… injected with PBS (control) or 100 µg of MOG 35-55 (New England Peptide) emulsified in 100 µl complete Freund’s adjuvant containing 300 g Mycobacterium tuberculosis (Fisher Scientific), and with 200 ng pertussis toxin (List Biological Laboratories), intraperitoneally …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

… Purified LPS from Escherichia coli was obtained from List Biological Laboratory Inc. (Campbell, CA, USA) and dissolved in endotoxin-free water. …

Author did not specify which List Labs E. coli LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… Pertussis toxin was purchased from List Biological Laboratories (Campbell, CA). …

Induction of EAE and Therapeutic Study:

EAE was induced as previously described … Additionally, 200 ng of pertussis toxin was administered as an intraperitoneal injection on the same day of immunization (day 0) as well as day 2 post-induction. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Chicken polyclonal IgY (753A) against TcdA was purchased from List Biological Labs. …