Citations

… overlaying the SC, or the lateral posterior thalamus. A glass pipette … containing either CTB (List Biological Laboratories, Campbell, CA; 1% in phosphate buffer 0.1 M) or PHA-L (2.5% PHA-L in 10 mM phosphate buffer, pH 8; …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… anti-CTB at 1:10,000 (List Biological Laboratories, Campbell, CA). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE:

EAE was induced in 7- to 13-wk-old female mice (Kihara et al., 2009). Briefly, mice were immunized subcutaneously with 150 µg myelin oligodendrocyte glycoprotein … in PBS and complete Freunds adjuvant containing 4 mg/mL M. Tuberculosis H37Ra (Difco) with or without intraperitoneal injection of 250 ng pertussis toxin (List Biological Laboratories) on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin was from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Indirect immunofluorescence assays:

HFF cells seeded on glass cover slips were infected with freshly isolated tachyzoites for 24 h, fixed with 4 % paraformaldehyde for 10 min, and then neutralized with 0.1 M glycine in PBS for 5 min. Samples were permeabilized by 0.1 % triton X -100 in PBS for 20 min, blocked with 2 % bovine serum albumin in PBS/0.1 % triton X -100 for 20 min. Subsequently, they were stained with primary antibodies (mouse anti -HA, 1:10.000; rabbit anti -TgGap45, 1:10.000) for 1 h and then with respective secondary antibodies (Alexa488 and Alexa594, 1:3.000) for 45 min in the dark. Samples were mounted into a mix of fluoromount G and DAPI, dried at 4 C overnight and examined by fluorescence microscopy (ApoTome, Zeiss). To resolve the C -terminal topology of TgFNT1 – 3, extracellular parasites were stained with a mouse -HA antibody (1:3000), suspended either in 3% BSA/PBS solution for pre -permeabilization staining, or in 2% BSA/0.2% triton X -100/PBS for post -permeabilization, as shown in Fig. S1. The inner membrane complex was separated from the plasma membrane by treating extracellular parasites with -toxin from Clostridium septicum (20 nM, 2 h) (List Biological Laboratories) followed by fixation on BSA -coated (0.01%) coverslips. In both cases, the standard secondary antibody staining procedure was performed afterwards, as described above.

… CTb Goat 1:200,000 List Biological 703 …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Pertussis toxin (200 ng, List Biological Laboratories) in PBS was administered i.v. on days 0 and 2. Mice were examined daily and scored for disease severity using the standard scale: 0, no clinical signs; 1, limp tail; 2, paraparesis (weakness, incomplete paralysis of one or two hind limbs); …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

All animal work was in compliance with our protocol approved by the University of British Columbia Animal Care Committee (certificate #A130281) and per the Canadian Council on Animal Care guidelines. Eight-week-old female C57BL/6 (Jackson Laboratories, Bar Harbor, ME) mice were immunized for chronic progressive EAE as previously described [41, 42] with 200 g of MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK; Stanford Pan Facility, Stanford, CA) in 4 mg/mL Mycobacterium tuberculosis (H37Ra) in incomplete Freunds adjuvant (IFA) (Difco Laboratories, Detroit, MI). Pertussis toxin (200 ng, List Biologicals, Campell, CA) was delivered intraperitoneally that day and 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… They then received a unilateral injection of 100 nl of 0.1% CTB (List Biological Laboraties, CA) diluted in water into either the NAc or VTA at the same coordinates in the opposite hemisphere, or the BLA (in mm relative to bregma, anteroposterior, -2.7, mediolateral, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction of cholangitis and UC-MSCT:

… Additionally, mice received 100 ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA) in 100 L PBS I.P. at the time of initial immunization with 2OA-BSA and 2 days afterwards, respectively. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)