Citations

Flow cytometry:

CD169 expression on MDMs was quantified by staining with Alexa647-conjugated mouse anti-human CD169 antibody (BioLegend, # 346006, 1:50) and analyzed with BD LSRII (BD). Geo mean fluorescence intensity (MFI) was calculated and normalized to that of uninfected (mock) MDMs. In some experiments, intracellular p24 was stained as described⁴⁹ using FITC- or RD1-conjugated mouse anti-p24 monoclonal antibody (KC57, Coulter, # 6604665 or # 6604667, 1:25). For T-cell exhaustion assay, MDMPBMC co-culture was harvested, stained with Zombie-NIR (BioLegend, #423105, 1:250) and a panel of makers designed for inhibitory receptor expression profiling⁵⁷, and analyzed with FACS ARIA (BD). For intracellular IFN- staining, MDMPBMC co-culture was unstimulated or stimulated with SEB (List Biological Laboratories) for 1h, followed by culture with brefeldin A (BioLegend) for another 5h, stained for inhibitory receptors as described above, fixed with FluoroFix Buffer (BioLegend), and stored in a staining buffer (2% NCS in PBS). Fixed cells were permeabilized with intracellular staining permeabilization wash buffer (BioLegend), stained with anti-human IFN- PE (BioLegend, # 506506, 1:200), and analyzed with FACS ARIA (BD). Data was analyzed with FlowJo software (FlowJo).

EAE:

… Pertussis toxin (List Biological Labs) was injected intraperitoneally (ip) on day 0 and day 2 of the immunization [6].  Mice were monitored each day for the development of clinical symptoms. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cancer Immunology, Immunotherapy 1 3 administered ip in 200 L of 0.4 mg/mL solution; mice were treated every 2 days beginning day 9, as described [22]. Diphtheria toxin (DT); (List Biological Laboratories, Campbell, CA; cat no 150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… LPS stock solution was purchased from List Biological Laboratories Inc., Campbell, California.  (ULTRA PURE LPS from Escherichia coli O55:B5) …

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

… Lipopolysaccharide (LPS), macrophage-activating lipopeptide of 2 kDa (MALP-2), and polyinosinic:polycytidylic acid (poly[I:C]) were purchased from List Biological Lab (Campbell, CA), Alexis Biochemical (San Diego, CA), and Amersham Biosciences …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… For footpad immunizations, mice were immunized once s.c. in the footpad with KLH48 (1 g/mouse), KLH + c-di-GMP (10 g/mouse), KLH + LP1569 (50 g/mouse) or KLH + LP-GMP or PBS. Alternatively, mice were immunized twice at 0 and 4 weeks with experimental aP vaccines, consisting of 2 or 3 B. pertussis antigens, FHA (Kaketsuken, Kumamoto, Japan), recombinant pertussis toxin (rPT; LIST Laboratories; #184) with or without PRN (LIST Laboratories; #187) formulated …

• Product #184 – Pertussis Toxin Mutant
• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

EAE induction:

EAE was induced in p38CD11c and p38fl/fl mice of both sexes at the age of 9-12 weeks as described earlier (Chechneva et al, 2011). In brief, mice were injected subcutaneously at two flank sites with an emulsion of 300 µg of MOG35-55 (New England Peptide) in incomplete Freund’s adjuvant supplemented with 500 µg Mycobacterium tuberculosis H37Ra (Difco). Mice received 200 ng of PTX (pertussis toxin) (List Biological Labs) intraperitoneally on day 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… pertussis toxin from List Biological Laboratories, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera toxin (CT) was obtained from List Biological Laboratories Inc, (Campbell, CA, USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Each mouse was immediately, thereafter, injected intraperitoneally and 48 h later with 200 ng pertussis toxin (List Biological Laboratories, Campbell, USA) in 100 µL 0.9% NaCl. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)