Bacterial Toxin Research Citations

… tracer Cholera toxin B fragment (CTB low salt, end concentration 0,03%) (List Biological Labs, Campbell, CA) was injected in a single dose (2 l delivered over 30 minutes) into was visualized using as primary antibody goat anti-choleragenoid (List Biological Labs …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE development and anti-IL-17 antibodies treatment:

… EAE was induced in 9-week-old female C57BL/6 mice. Mice were immunized subcutaneously with an emulsion containing myelin oligodendrocyte glycoprotein(MOG) 3555 peptide (synthesized in-house) and Complete Freund’s Adjuvant (CFA). Immunization was performed under isoflurane anesthesia. All animals received pertussis toxin (List Biological Laboratories, Campbell, CA) in PBS intraperitoneally (i.p.) on day 0 and day 2 after MOG immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice also received 400 ng of pertussis toxin (LIST Biological Laboratories) intraperitoneally both immediately after immunization and 48 h later. Control mice were injected with everything except the MOG peptide …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Recombinant antigens and coupling to microsphere beads:

… Tetanus toxoid (Massachusetts Biological Laboratories, Boston, MA), diphtheria toxoid from Corynebacterium diphtheriae (List Biological Laboratories, Campbell, CA), and recombinant measles nucleoprotein (MV-N, Meridian Life Sciences, Memphis, TN) [30] were purchased from commercial sources. Tetanus toxoid was coupled to SeroMap beads as previously described [31]. Diphtheria toxoid was coupled in buffer containing 50 mM 2-(N-morpholinoethanesulfonic acid (MES) at pH 5.0 with 0.85% NaCl at a concentration of 60 g of protein per 1.25 10⁷ beads in 1 ml final volume. …

• Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae

… Diphtheria Toxin (unnicked) List Biological Laboratories Cat # 150 …

• Product #150 – Diphtheria Toxin, Unnicked, fromCorynebacterium diphtheriae

LPS:

Purified LPS from Salmonella Typhimurium (catalog no. 225) was obtained from List Biological Laboratories, Inc. (Campbell, CA) …

• Product #225 – LPS from Salmonella typhimurium

… Pertussis toxin was purchased from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera toxin was from List Biological Laboratories (cat. no. 100B). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Lactate dehydrogenase (LDH) assay:

Macrophages were pretreated for 30min with glycine or one of the other small-molecule inhibitors at the indicated dose. All chemicals were purchased from Sigma-Aldrich, with the exception of propofol (EMD Millipore). Late-log cultures of Salmonella typhimurium SL1344 grown in L-broth containing 0.3M NaCl were used to infect macrophages (MOI 10:1) for 90min, unless otherwise indicated. Cells were treated with anthrax lethal toxin comprised of 1g/ml protective antigen and 1g/ml lethal factor (List Biological) for 2h¹⁴. Cells were treated with the detergent-based lysis buffer included in the Cytotox 96 Kit (Promega) for 30min. Release of cytoplasmic LDH was determined from triplicate samples using the Cytotox 96 Kit and calculated as 100 (experimental LDHspontaneous LDH)/(maximum LDHspontaneous LDH).

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

… biological sample (S. aureus) Staph enterotoxin B List Biological Laboratories #122 …

Mixed lymphocyte cultures:

Mouse splenic CD4⁺ T cells were purified with a Stem Cell Technologies negative selection CD4⁺ T cell isolation kit and co-cultured with bone marrow-derived dendritic cells (cultured with GM-CSF for 7 days) at 1:1 ratio with or without Staphylococcal entertoxin B (SEB, 0.05 g/ml, List Biological Laboratories,>95% pure, 14 EU/mg) and in the presence of either IL-21R-Fc blocking agent or control Fc (20 g/ml) for 24 hr; RNA was then isolated. For intracellular staining of IL-21, human CD4⁺ T cells were purified from peripheral blood (Dynabeads Untouched negative selection CD4⁺ T cell kit, Invitrogen) and co-cultured at a 5:1 ratio with monocyte-derived DCs that had been cultured for 6 days in GM-CSF (800 U/ml) plus IL-4 (400 U/ml). At 72 hr, cultures were treated with Golgi Plug for 4 hr and stained