Bacterial Toxin Research Citations

… LF (List Labs), …

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

2.2 Stereotaxic NAc injections

2.2.1 CTb or red retrobeads in mice

TH-GFP mice were initially anaesthetized with 5% isoflurane (Abbott Park, Illinois, USA) in air, and maintained with 2–3% isoflurane in air as necessary. The retrograde tracers using a Picospritzer; cholera toxin B subunit (CTb, 20–80 nL, 1% in distilled water; List Biological Laboratories; RRID:AB_10013220) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… pertussis toxin (List Biological, #181), …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Induction of active experimental autoimmune encephalomyelitis

… All emulsions or mixtures were then injected s.c. into the flank skin of mice on days 0 and 7 for the 2×MOG immunization. In addition, pertussis toxin (PTX; 0.2 μg/mouse; 181; List Biological Laboratories, Inc.) was injected i.v. on days 0, 2, and 7. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Induction of EAE in DR2-Tg mice

HLA-DR2 mice were screened by FACS for the expression of the HLA transgenes. HLA-DR2 positive male mice between 8 and 12 weeks of age were immunized s.c. at two sites on the flanks with 0.2 ml of an emulsion of 100-200 μg immunogenic peptide and complete Freund’s adjuvant containing 400 μg of heat-killed Mycobacterium tuberculosis H37RA (Difco, Detroit, MI). In addition, mice were given pertussis toxin (Ptx) from List Biological Laboratories (Campbell, CA) on days 0 and 2 post- immunization (2* 200 ng per mouse). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Vaccine composition for PT mRNA antigen optimization

DTaP (Infanrix; GSK) and wP (DTP; Serum Institute of India) were diluted to 1/20th of a single human dose. gPT was obtained through List Biological laboratories [cat#184 (50 µg)] …

Pertussis toxin challenge model

Purified pertussis toxin [List Biological laboratories; cat#180 (50 µg)] was reconstituted in 500 µL of endotoxin-free 1× PBS to yield a concentration of 0.1 µg/ µL.

Serological survey for PT antigen optimization

IgG antigen-specific antibodies in vaccinated and unvaccinated mice were measured via an ELISA. Plates were coated with 50 µL per well of either whole PT (List Biological Laboratories; cat#180)…

• Product #184 – Pertussis Toxin Mutant

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE

EAE was induced in 8-12-week female C57Bl/6J mice using 150 μg of MOG35–55 (Genemed Synthesis, 110582) mixed with freshly prepared complete Freund’s Adjuvant (using 20 ml Incomplete Freund’s Adjuvant (BD Biosciences, #BD263910) mixed with 100 mg Myobacterium tuberculosis H37Ra (BD Biosciences, #231141)) at a ratio of 1:1 (v/v at a concentration of 5 mg/ml). All mice received two subcutaneous injections of 100 μl each of the MOG35-55/CFA mix. All mice then received a single intraperitoneal injection of 200 ng pertussis toxin (List Biological Laboratories, #180) at a concentration in 200 μl of PBS. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… cholera toxin (CT) (List Labs, CA, USA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Most of the pertussis toxins used in this study were purchased from List Biological Laboratories, Campbell, CA, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… To induce myocarditis, IL-5tg received subcutaneous immunizations on days 0 and 7 of 100 µg myosin heavy chain α (MyHCα) 614 peptide (Ac-SLKL MATL FSTY ASAD; Genscript), or 790 peptide (Ac-IQAQ ARGQ LMRI EFKK)15 emulsified in complete Freund’s adjuvant (CFA, Sigma-Aldrich) supplemented to 5 mg/ml heat-killed Mycobacterium tuberculosis strain H37Ra (BD Biosciences). On day 0, mice also received 500 ng pertussis toxin intraperitoneally (List Biologicals) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)