Bacterial Toxin Research Citations

… Targeted elimination of CX3CR1+ cells. CX3CR1-DTR mice were treated with diphtheria toxin (DT, List Biological Lab Inc., Campbell, CA) three times over seven days using a dose of 200ng/20g mouse. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Drugs:

The following compounds were used in the reported studies: CL 316,241 (in saline; from Tocris); C75 (RPMI medium 1640; from Tocris);67,68 capsaicin (3.3% Tween-80 in saline; from Sigma)14, CNO (in saline; from Enzo Life Science)14, and diphtheria toxin (in saline; List Biological, cat. no. 150). All drugs were injected in a volume of 10 ml kg1 of body weight i.p. except for diphtheria toxin, which was injected at the dose of 50 g kg1 and the volume was 2.66 l g1 of body weight. When multiple injections were performed in the same experiment, the volume of each injection was adjusted to a total volume of 10 ml kg1 per animal.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Experimental Animal Procedures:

All mice were intragastrically (i.g.) sensitized to the cows milk protein whey (DMV International, Veghel, the Netherlands) dissolved in PBS (20 mg whey in 0.5 ml PBS, Lonza, Verviers, Belgium) with cholera toxin (CT, 15 µg CT in 0.5 ml, List Biological Laboratories Inc., Campbell, CA, USA) to induce food allergy or were sham-sensitized with PBS and CT alone on D0, 7, 14, 21, and 28 (Figure 1). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Animals received 250 µg pertussis toxin (List Biological Laboratories) intraperitoneally (I.P.) on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Recombinant anthrax lethal factor was purchased from List Biological Laboratories, Inc. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169L (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

EAE induction

Mice were immunized subcutaneously in two sites (left and right flanks) with 150 μg of PLP 139–151 (New England Peptide, Gardner, MA) peptide that was emulsified in complete Freund’s adjuvant (CFA; Sigma-Aldrich) containing 200 μg Mycobacterium tuberculosis (Difco), followed by 200 ng pertussis toxin (PT; List Biological Laboratories) in 0.2 ml PBS by intraperitoneal (i.p.) injections at the time of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… After 30min, mice were given 20mg BLG (Sigma-Aldrich) plus 10µg cholera toxin (List Biologicals). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… For experiments with TLR7.1/Ccr2- DTR mice, mice were injected intraperitoneally with 10 ng DT/g of body weight (List Biological Laboratories) in phosphate-buffered saline every other day for 17 days.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Retrograde tracer injections in nucleus reuniens and the mammillary bodies:

A total of ten rats received retrograde tracer injections. In seven of these cases, two retrograde tracer injections were made, one directed at nucleus reuniens, the other at the mam-millary bodies (Table 1). In the three remaining cases only the injection directed at nucleus reuniens was successful. Different tracers were used, one in each site. The tracers used were fast blue (FB, SigmaAldrich, Gillingham, UK) and choleratoxin b (CTB, List Biological Laboratories Inc, CA; 1% solution in 0.05 M tris). Except for one CTB injection that was injected by iontophoresis, all retrograde tracers were injected mechanically at a rate of 20 nl/min via a 0.5 µl or 1.0 µl Hamilton pipette (Hamilton, Bonaduz, Switzerland). Individual injection volumes for mechanical injections were 50-60 nl. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Test approach:

No standardized approaches are available to investigate anthrax seroprevalence in wildlife. Thus, samples were tested for antibodies against the anthrax protective antigen (PA, List Biological Laboratories Inc) using an in-house ELISA and an in-house Western Blot and for antibodies against anthrax lethal factor (LF, List Biological Laboratories Inc) using an in-house Western Blot. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169L (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis