Citations

Breakdown of BBB and Passive Transfer of Human IgG to Mice:

Female wild-type C57BL/6 mice of age 68 weeks were used. …

Procedures of the animal experiments were summarized in Figure 1A. … In addition, mice received two intraperitoneal (i.p.) injections of pertussis toxin (PTx, List Biological Laboratories, USA), 200 ng in 0.2 ml PBS each, 7 and 3 days before IgG transfer. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 2.2. Dose regimens. Currently, the only commercially available LPS for use in humans is the GMP-grade E. Coli Type O113 (Lot no. 94332) produced by List Biological Laboratories in Campbell, USA. …

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

… The cells were washed and incubated with serum-free medium for 1 hour. The pertussis toxin (PTX, List Biological Laboratories Inc., Campbell, CA) was added to the cell medium at 0.2 mg/ml and allowed reaction for 1 hour in the incubator …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE and treatment:

… Pertussis Toxin (PTX, List Biological Laboratories, CA, USA) was injected i.p. at the time of immunization and 48h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Multiplexed Luminex Immunoassays:

Antigen-specific antibody responses were measured through a modified multiplexed Luminex assay (89). Pertussis and tetanus proteins (Pertussis Toxin Mutant; PT, Pertactin; PRN, … Fimbriae2/3; FIM2/3, Adenylate Cyclase Toxin; ACT and Tetanus toxoid; TT from List Biological Laboratory, Campbell, CA …

• Product #186 – Fimbriae 2/3 from B. pertussis
• Product #187 – Pertactin from B. pertussis (69 kDa Protein)
• Product #184 – Pertussis Toxin Mutant
• Product #188L – Adenylate Cyclase Toxin, Recombinant from B. pertussis
• Product #190 – Tetanus Toxin from Clostridium tetani
• Product #191 – Tetanus Toxoid from Clostridium tetani

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EE:

… Additionally, mice were administered 75 ng of pertussis toxin (Ptx, List Biologicals, Campbell, CA) via an intraperitoneal (ip) injec- tion on the day of immunization and 200 ng ip two days later. All mice were monitored daily for weight loss and clinical signs of EAE disease …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental design:

… In addition, mice received intravenous pertussis toxin (List Biological Laboratories, Campbell, CA, USA) on Days 0 and 2. Control mice were treated with complete Freund’s adjuvant and saline alone.

Experiment 2:

To examine the effect of delayed treatment with MR16-1 for a longer period than in Experiment 1, EAE induction method was modified. EAE mice treated with 50 µg MOG₃₅₅₅ and 250 ng pertussis toxin were intraperitoneally administered MR16-1 (8mg/mouse) or vehicle on Day 12. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunization:

For mucosal immunization, mice were anesthetized with isoflurane and then intrarectally administered 40 µl of sterile PBS containing 10 µg cholera toxin (List Biological Laboratories, Inc.; Campbell, CA) and …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Measuring the LPS induced response in IC-21 cells:

A 12-plex mouse inflammatory cytokine multi-analyte ELISArray kit (Qiagen) paired with LPS O111:B4 (phenol extract, Sigma Aldrich) was used to determine how many hours of LPS exposure was required to produce a measurable cytokine response. Limit of detection for the ELISAarray 12-plex kit is stated by the manufacturer as 10 pg/mL for each given cytokine. For further in-depth examination we chose ULTRA PURE LPS O111:B4 (List Labs) to avoid contaminates often found in other LPS preparations. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Reagents:

…Pertussis Toxin (PTX) from List Biological …

Results:

… For each ligand, inhibition of cAMP accumulation was blocked by pretreatment with PTX (100 ng/mL), indicating that these were Gi-mediated effects (octreotide not shown) (Fig. 1C and 1D). These results demonstrate that in pituitary tumor cells, both octreotide and SOM230 had full efficacy for cAMP inhibition and this effect was fully inhibited by PTX. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)