Bacterial Toxin Research Citations

Antibodies:

Primary antibodies used in this study that are commercially available include: rabbit anti-MEK2 (Santa Cruz Biotechnology sc-523, AB_2281672), goat antiprotective antigen from Bacillus anthracis(List Biological Laboratories #771B), …

LFNEffector intoxication of T84 cells:

… For intoxication of cells, media was replaced over 105 cells previously seeded in a 12-well plate. 31.7 nM PA alone (List Labs, #171E) or in the presence of 13.6nM LFN, LFN-ACD, or LFN-RID was added to media and cells were incubated for 20 hr at 37C in the presence of 5% CO2. For co-intoxication of 13.6 nM LFNACD and LFN-RID, 3x excess PA was used to ensure equal translocation of both effectors.

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… For the induction of MP4-EAE, mice received 200 µg MP4 (Alexion Pharmaceuticals Inc) emulsified in CFA and 200 ng pertussis toxin (List Biological Laboratories, #181, Lot #18123611) in 500 µl sterile phosphate-buffered saline (PBS) on the day of immunization …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Targeted elimination of CX3CR1+ cells. CX3CR1-DTR mice were treated with diphtheria toxin (DT, List Biological Lab Inc., Campbell, CA) three times over seven days using a dose of 200ng/20g mouse. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Drugs:

The following compounds were used in the reported studies: CL 316,241 (in saline; from Tocris); C75 (RPMI medium 1640; from Tocris);67,68 capsaicin (3.3% Tween-80 in saline; from Sigma)14, CNO (in saline; from Enzo Life Science)14, and diphtheria toxin (in saline; List Biological, cat. no. 150). All drugs were injected in a volume of 10 ml kg1 of body weight i.p. except for diphtheria toxin, which was injected at the dose of 50 g kg1 and the volume was 2.66 l g1 of body weight. When multiple injections were performed in the same experiment, the volume of each injection was adjusted to a total volume of 10 ml kg1 per animal.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Experimental Animal Procedures:

All mice were intragastrically (i.g.) sensitized to the cows milk protein whey (DMV International, Veghel, the Netherlands) dissolved in PBS (20 mg whey in 0.5 ml PBS, Lonza, Verviers, Belgium) with cholera toxin (CT, 15 µg CT in 0.5 ml, List Biological Laboratories Inc., Campbell, CA, USA) to induce food allergy or were sham-sensitized with PBS and CT alone on D0, 7, 14, 21, and 28 (Figure 1). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Animals received 250 µg pertussis toxin (List Biological Laboratories) intraperitoneally (I.P.) on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Recombinant anthrax lethal factor was purchased from List Biological Laboratories, Inc. …

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

EAE induction

Mice were immunized subcutaneously in two sites (left and right flanks) with 150 μg of PLP 139–151 (New England Peptide, Gardner, MA) peptide that was emulsified in complete Freund’s adjuvant (CFA; Sigma-Aldrich) containing 200 μg Mycobacterium tuberculosis (Difco), followed by 200 ng pertussis toxin (PT; List Biological Laboratories) in 0.2 ml PBS by intraperitoneal (i.p.) injections at the time of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… After 30min, mice were given 20mg BLG (Sigma-Aldrich) plus 10µg cholera toxin (List Biologicals). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae