Bacterial Toxin Research Citations

Diphtheria toxin List Biological Labs Part# 150

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Quantification of R-CTB and R-CTB by densitometry:

Known concentrations of purified CTB (List Laboratories) were resolved by SDS-PAGE alongside R-CTB or R-CTB samples (plasmid and chromosomal) and used as standards for quantification. ImageJ was used to generate a standard curve fitted to the intensities of bands corresponding to the CTB standards. The concentrations of R-CTB and R-CTB were calculated using the linear portion of the standard curve.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Materials:

… List Labs , #703; …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Breakdown of BBB and Passive Transfer of Human IgG to Mice:

Female wild-type C57BL/6 mice of age 68 weeks were used. …

Procedures of the animal experiments were summarized in Figure 1A. … In addition, mice received two intraperitoneal (i.p.) injections of pertussis toxin (PTx, List Biological Laboratories, USA), 200 ng in 0.2 ml PBS each, 7 and 3 days before IgG transfer. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 2.2. Dose regimens. Currently, the only commercially available LPS for use in humans is the GMP-grade E. Coli Type O113 (Lot no. 94332) produced by List Biological Laboratories in Campbell, USA. …

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

… The cells were washed and incubated with serum-free medium for 1 hour. The pertussis toxin (PTX, List Biological Laboratories Inc., Campbell, CA) was added to the cell medium at 0.2 mg/ml and allowed reaction for 1 hour in the incubator …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE and treatment:

… Pertussis Toxin (PTX, List Biological Laboratories, CA, USA) was injected i.p. at the time of immunization and 48h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Multiplexed Luminex Immunoassays:

Antigen-specific antibody responses were measured through a modified multiplexed Luminex assay (89). Pertussis and tetanus proteins (Pertussis Toxin Mutant; PT, Pertactin; PRN, … Fimbriae2/3; FIM2/3, Adenylate Cyclase Toxin; ACT and Tetanus toxoid; TT from List Biological Laboratory, Campbell, CA …

• Product #186 – Fimbriae 2/3 from B. pertussis
• Product #187 – Pertactin from B. pertussis (69 kDa Protein)
• Product #184 – Pertussis Toxin Mutant
• Product #188L – Adenylate Cyclase Toxin, Recombinant from B. pertussis
• Product #190 – Tetanus Toxin from Clostridium tetani
• Product #191 – Tetanus Toxoid from Clostridium tetani

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EE:

… Additionally, mice were administered 75 ng of pertussis toxin (Ptx, List Biologicals, Campbell, CA) via an intraperitoneal (ip) injec- tion on the day of immunization and 200 ng ip two days later. All mice were monitored daily for weight loss and clinical signs of EAE disease …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental design:

… In addition, mice received intravenous pertussis toxin (List Biological Laboratories, Campbell, CA, USA) on Days 0 and 2. Control mice were treated with complete Freund’s adjuvant and saline alone.

Experiment 2:

To examine the effect of delayed treatment with MR16-1 for a longer period than in Experiment 1, EAE induction method was modified. EAE mice treated with 50 µg MOG₃₅₅₅ and 250 ng pertussis toxin were intraperitoneally administered MR16-1 (8mg/mouse) or vehicle on Day 12. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)