Bacterial Toxin Research Citations

Assessment of Glutamate Release from Rat Cortical Neurons Treated with Botulinum Neurotoxin:

Glutamate release was assessed in cortical neurons at DIV 1921. Cortical neurons were treated with a concentration-range of nBoNT/A (LIST Laboratories, Campbell, CA, USA), …

Measurement of memory B cell responses:

… On the day prior to testing for MBC, 96-well ELISPOT plates (Millipore MIPS4150) were coated overnight at 4 °C with antigen or capture antibody. Coating antigen consisted of LPS from V. cholera, Inaba 569B serotype (University of Maryland Center for Vaccine Development; 50 µg/mL) or cholera toxin B (CtxB) (List Biological Laboratories; 15 µg/mL). Assay wells receiving CtxB were first pre-coated with GM1 ganglioside (Enzo Life Sciences; 3 nM) for 3 h at room temperature. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Escherichia coli lipopolysaccharide (O55:B5) was purchased from List Biologicals (Campbell, CA, USA), and prepared in phosphate-buffered saline. …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

EAE induction and treatment protocol:

Chronic EAE was induced in 68 weeks old C57Bl/6 mice by subcutaneous immunization with 300µg of MOG3335 peptide in complete Freuds adjuvant (Becton Dickinson) containing 0.8mg/ml Mycobacterium Tuberculosis (Becton Dickinson), as previously described42,43. Mice were also injected intravenously (i.v.) with pertussis toxin (40ng; List Biological Laboratories) at the day of immunization and after 48hours. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials:

… Tetanus toxoid from Clostridium tetani List Biological Laboratories …

• Product #191 – Tetanus Toxoid from Clostridium tetani

Reagents and Antibodies:

… Anthrax lethal toxin was purchased from List Biological Laboratories, …

LDH cytotoxicity assays:

RAW 264.7 cells were seeded at 0.5 106 cells/well in 6-well plates in 15 
standard growth medium 24 hours prior to treatment. Cells were treated with the indicated agents
for the indicated times. Supernatants were then harvested and analyzed for LDH activity using an
LDH cytotoxicity assay kit (Pierce).  For experiments with proteasome inhibitors or bestatin
methyl ester, cells were treated with these agents 30 minutes prior to the addition of Val-boroPro
and/or LT, and then incubated for the indicated amount of time. …

Immunoblotting experiments:

RAW 264.7 macrophages were seeded at 0.5 106 cells/well in
6-well plates or at 5 106 cells/dish in 10 cm plates, and 24 h later were treated with agents as
described. Bestatin methyl ester and bortezomib, if used, were added 30 minutes prior to LT …

Immunoprecipitation experiments:

For immunoprecipitation experiments, HEK 293T cells were
seeded at 7 106 cells/well in 10 cm plates and transiently transfected with 5 g of N-terminal V5
and C-terminal FLAG-tagged Nlrp1b and 5 g of N-terminal HA-tagged ubiquitin using Fugene 10 
HD. After 24 h, cells were then treated with bortezomib for 30 minutes followed by LT or vehicle
for an additional 3 hours and harvested. …

Anthrax Lethal Toxin (LT) = Anthrax Lethal Factor (LF) + Anthrax Protective Antigen (PA)

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… SEB-stimulated (0.5 g/ ml; List Biological Laboratories) and unstimulated samples for each animal were included. …

Blood-brain barrier permeability assay:

Permeability across the blood-brain barrier was studied using a noninvasive whole body imaging InVivoXtreme 4MP (BRUKER, formerly Carestream). Brain permeability was assessed ex situ 24 hours after treatment in either control S. pneumoniaeinfected or pertussis toxin treated animals (200 ng/d from B. pertussis for 2 consecutive days treated as a positive control [#180 Islet activating protein, List Biological Laboratories]). …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Effect of αDCIR2-PLP139–151 fusion mAbs on the induction of EAE

To determine the therapeutic effect of DCIR2+ DC targeting on the clinical outcome of EAE, cohorts of 6 to 10-week-old female SJL/J mice were either left untreated or injected i.p. with 1μg of fusion mAbs (αDCIR2-PLP139–151 mAb, isotype control/ PLP139–151 mAb) 10 days before inducing EAE. Where indicated, cohorts of control mice were injected with 1 μg of conventional αDCIR2 mAb. For EAE induction, SJL/J females were immunized s.c. with 75 μg of synthetic PLP139–151 peptide emulsified in CFA. Pertussis toxin (200 ng, List Biological Laboratories, Campbell, CA) was administered i.v. on the next day. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The CTb antibody (1:10,000, List Biological, cat#703) was a goat polyclonal raised against choleragenoid. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)