Bacterial Toxin Research Citations

Reagents:

… Ultrapure LPS (Escherichia coli 0111: B4) was purchased from List Biological Laboratories (Vandell Way, CA, USA). This LPS does not contain contaminating proteins that could stimulate TLR2 nonspecifically. …

Experimental model:

C57BL/6 male mice at 8-10 weeks of age were used for the present experiments. Mice were housed in a temperature- and humidity-controlled environment on a constant 12:12-h light-dark cycle. All animal studies were approved by the Animal Ethics Committee of Jinzhou Medical University. Mice were fasted for 12 h with water ad libitum before the operation. Mice were anesthetized with 2% isoflurane, and common BDL was performed as previously described [26]. In brief, following a peritoneal cavity incision, the common bile duct was dissected and double-ligated with a 5-0 silk suture, and then the bile duct was cut between the ligatures. Controls underwent a sham operation in which the common bile duct was exposed but not ligated. A two-layer running suture was used for abdominal closure with 4-0 dexon and 2-0 nylon. Two concentrations of DA (25 or 50 mg/kg) were administered intraperitoneally daily at 9:00 AM beginning after the ligature on day 1. Four days after BDL, selected mice were subjected to an ileocecal vein injection of LPS under microscopy at a dose of 100 ng/ gram of body weight (gbw), 1 g/gbw or 5 g/gbw diluted with 300 l of PBS.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

… After 233 hours in culture, cells were exposed to UltraPure LPS (List Biologicals, Campbell, CA) at 234 g/ml for 24 hours before harvesting and phenotyping …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… current pulses, 7 s duration every 7 s for 10 min) of the retrograde tracer cholera toxin subunit b – CTb (low salt, 1% in distilled water, List Biological Laboratories, Campbell, USA). CTb was detected using a goat anti-CTb antibody (1:2000, List Biological …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Perfusions and tissue sectioning:

… Freefloating sections were immunostained for CTb, NeuN or cFos by overnight incubation in goat antiCTb (List Biological Laboratories, 7032A9, 1: 50,000 dilution), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… The mice were also injected with intravenous Bordetella pertussis toxin (200 ng; PTX, List Biological Laboratories Inc.) in 1X Phosphate Buffered Saline (PBS) at the time of and two days following immunization …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For the detection of GM1 ganglioside, biotinylated cholera toxin B subunit (1:1000; List Biological Laboratories, Inc., Campbell, CA, USA) was used. …

• Product #112 – Cholera Toxin B Subunit Biotin Conjugate

… The primary antibodies used were guinea pig anti-oxytocin 1:5,000 (OXT; Peninsula Laboratories, San Carlos, CA, USA) and goat anti-CTB 1:100,000 (List Biological Labs, Campbell, CA, USA). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Injection site visualization:

… In the other cases (S4-5, S11-13 and S17-18), the sections were first rinsed with PB 0.1M and then permeated with PB 0.1 M and 0.3 % Triton X-100. They were then incubated overnight in a choleragenoid antibody raised in goat (goat anti-CTb, lot no. 703, List Biological Laboratories, USA) diluted 1: 2000 in a blocking solution (PB 0.1 M, 5 % BSA). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Microinjection of CTB. Under general anaesthesia with isoflurane, a total of 3 µL 1% CTB (List Biological Labs, Campbell, California, USA) was used for injections at GB14, ST2 and ST6 in different rats (n=3 for each acupuncture point) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EXPERIMENTAL PROCEDURES

Antigens and antibodies:

PTx (holotoxin) in glycerol was purchased from List Biological Laboratories, Inc. (Campbell, CA) …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)