Bacterial Toxin Research Citations

MOG immunization and EAE:

Mice were immunized with 100 mg of myelin oligodendrocyte glycoprotein (MOG)3555 peptide in CFA (Difco) with 500 mg of Mycobacterium tuberculosis (Difco). Mice were i.p. injected 200 ng of pertussis toxin (List Biological,#181) on the day of immunization and 2 days later, as described (Kang et al., 2010). The mice were observed daily for clinical signs and scored as described previously (Shi et al., 2011).

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Reagents:

… Ultrapure LPS (Escherichia coli 0111: B4) was purchased from List Biological Laboratories (Vandell Way, CA, USA). This LPS does not contain contaminating proteins that could stimulate TLR2 nonspecifically. …

Experimental model:

C57BL/6 male mice at 8-10 weeks of age were used for the present experiments. Mice were housed in a temperature- and humidity-controlled environment on a constant 12:12-h light-dark cycle. All animal studies were approved by the Animal Ethics Committee of Jinzhou Medical University. Mice were fasted for 12 h with water ad libitum before the operation. Mice were anesthetized with 2% isoflurane, and common BDL was performed as previously described [26]. In brief, following a peritoneal cavity incision, the common bile duct was dissected and double-ligated with a 5-0 silk suture, and then the bile duct was cut between the ligatures. Controls underwent a sham operation in which the common bile duct was exposed but not ligated. A two-layer running suture was used for abdominal closure with 4-0 dexon and 2-0 nylon. Two concentrations of DA (25 or 50 mg/kg) were administered intraperitoneally daily at 9:00 AM beginning after the ligature on day 1. Four days after BDL, selected mice were subjected to an ileocecal vein injection of LPS under microscopy at a dose of 100 ng/ gram of body weight (gbw), 1 g/gbw or 5 g/gbw diluted with 300 l of PBS.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

… After 233 hours in culture, cells were exposed to UltraPure LPS (List Biologicals, Campbell, CA) at 234 g/ml for 24 hours before harvesting and phenotyping …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… current pulses, 7 s duration every 7 s for 10 min) of the retrograde tracer cholera toxin subunit b – CTb (low salt, 1% in distilled water, List Biological Laboratories, Campbell, USA). CTb was detected using a goat anti-CTb antibody (1:2000, List Biological …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Perfusions and tissue sectioning:

… Freefloating sections were immunostained for CTb, NeuN or cFos by overnight incubation in goat antiCTb (List Biological Laboratories, 7032A9, 1: 50,000 dilution), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… The mice were also injected with intravenous Bordetella pertussis toxin (200 ng; PTX, List Biological Laboratories Inc.) in 1X Phosphate Buffered Saline (PBS) at the time of and two days following immunization …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For the detection of GM1 ganglioside, biotinylated cholera toxin B subunit (1:1000; List Biological Laboratories, Inc., Campbell, CA, USA) was used. …

• Product #112 – Cholera Toxin B Subunit Biotin Conjugate

… The primary antibodies used were guinea pig anti-oxytocin 1:5,000 (OXT; Peninsula Laboratories, San Carlos, CA, USA) and goat anti-CTB 1:100,000 (List Biological Labs, Campbell, CA, USA). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Injection site visualization:

… In the other cases (S4-5, S11-13 and S17-18), the sections were first rinsed with PB 0.1M and then permeated with PB 0.1 M and 0.3 % Triton X-100. They were then incubated overnight in a choleragenoid antibody raised in goat (goat anti-CTb, lot no. 703, List Biological Laboratories, USA) diluted 1: 2000 in a blocking solution (PB 0.1 M, 5 % BSA). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Microinjection of CTB. Under general anaesthesia with isoflurane, a total of 3 µL 1% CTB (List Biological Labs, Campbell, California, USA) was used for injections at GB14, ST2 and ST6 in different rats (n=3 for each acupuncture point) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt