Bacterial Toxin Research Citations

… Additionally, they received 150 ng PT (List laboratories) on days 0 and 2 after immunization. To induce EAE in RAG1 KO mice, CD4+ T cells were isolated from the spleen and lymph nodes of 2D2 mice. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Serological analysis of B. pertussis specific antibodies

Serological responses to vaccination were quantified by ELISA. High-binding microtiter plates were coated with PT (50 ng/well) (PT#180, LIST Biologicals), as described in Boehm et al.20. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Pertussis toxin List Biological Laboratories Cat#: 180 …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Pertussis toxin (PTX; Cat#82248-93-9, List Biologicals, Campbell, CA, USA,) was administered intraperitoneally …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Peptide-based EAE Induction and Scoring

Approximately five male and five female mice, 8-14 weeks old, of each of the 32 CC strains were immunized
subcutaneously with 200 µg MOG35-55 (New England Peptide) emulsified in CFA (Sigma), supplemented with
additional 4 mg/ml heat-killed Mycobacterium tuberculosis (Difco Laboratories), and received a single
intraperitoneal injection of 200 ng PTX (List Biological Laboratories) on D0 as an ancillary adjuvant. Mice were
studied across a total of four cohorts. Five male and five female B6 mice per cohort, immunized as above, served
as reference controls for cohort effects and EAE induction.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Lipopolysaccharide (LPS, H2O, 1 µg/mL) List Biological Labs product #434 …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

hCEC Isolation From Human Corneas

Human corneas, which were unsuitable for transplantation, were obtained from the Veneto Eye Bank Foundation (Venice, Italy) and used for research purposes, according to Italian legislation and guidelines set by the Italian Transplant Service (Centro Nazionale Trapianti, Rome, Italy) and the Regional Transplant Service (Centro Regionale Trapianti, Padua, Italy). To isolate hCECs from human corneas, limbal tissue was dissected from human corneas, cut into small pieces, and incubated with 10 mL trypsin (0.05% trypsin and 0.01% EDTA solution, Life Technologies, 25300-062) for 30 minutes in 37°C, after which the supernatants, containing the cells, were collected, and neutralized by addition of 10 mL KM media containing: Dulbecco’s Modified Eagle Medium (21969035, Gibco) and F12 (21765029, Gibco) 2:1, fetal bovine serum (FBS) 10% (10099-141, Life Technologies), penicillin-streptomycin 50 mg/mL (15140122Life, Technologies), and Glutamine 4 mM (25030081, Life Technologies). Adenine grade I 0.18 mM, (4010-21-2, Pharma Waldhof GMBH), hydrocortisone 0.4 mg/mL (AIC013986029, Flebocortid Richter, Sanofi), insulin (HI0210, Humulin R, Lilly, Canada), Triiodothyronine 2 nM (AIC036906016, Liotir, IBSA), Cholera Toxin QD 8.1 mg/mL, 9100B, List Biological Laboratories), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Please reach out to List Labs for a custom order of product #9100B – Cholera Toxin QD at info@listlabs.com

2.1. Materials

… The following antigens were used for antigen-specific Luminex assays: Adenylate Cyclase Toxin [ACT] and Pertactin [PRN] were obtained from List Biological Laboratories (Campbell, CA, USA), …

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)
• Product #188L – Adenylate Cyclase Toxin, Recombinant from B. pertussis

… MOG35-55 with complete Freund’s adjuvant (Difco, Detroit, MI) containing 5 mg/ml heat-inactivated H37Ra (Difco, Detroit, MI). Additionally, Pertussis toxin (200 ng) (List Biological Laboratories, Campbell, CA) in PBS were administered i.p. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Diphtheria toxin treatment and adoptive transfer of Treg cells to Foxp3DTR mice

For the Treg cell depletion studies, Foxp3DTR mice were intra-peritoneally injected with a diphtheria toxin (DT, List Biologicals cat. no. 150) loading dose (50 μg/kg) on day 14 post-influenza virus inoculation and given three maintenance doses (10 μg/kg) every other day thereafter to deplete endogenous Treg cells. For the adoptive transfer experiments, Foxp3DTR mice received a DT loading dose one day before influenza virus inoculation as well as 1 x 106 Treg cells via retro-orbital adoptive transfer from either Black 6 or TBET KO mice enriched using the Miltenyi Biotec CD4+ CD25+ Regulatory T Cell Isolation Kit (cat. no. 130-091-041) according to the manufacturer’s instructions. Foxp3DTR mice receiving adoptive transfers were then given a DT maintenance dose every other day until tissue harvest.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae