Bacterial Toxin Research Citations

In vitro diphtheria toxin protection assay:

Mouse serum samples were first diluted 1:10 in RPMI containing 10% FBS and then serially diluted in 384-well plates using Bio-Tek Precision 2000 liquid-handlers (Bio-Tek, Winooski, VT). Forty L of 100 ng/mL Diphtheria toxin (List Biological Laboratories, Campbell, CA) was added to each sample well and incubated for 1h at 37C. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Bone marrow derived macrophage culture and TLR stimulation:

Bone marrow was harvested from 6- to 8-week-old male C57Bl/6N or Ifi35^-/- mice and cultured in macrophage growth media consisting of DMEM, 10% FBS, 10% M-CSF containing CMG-L929 conditioned media, 1% penicillin/streptomycin, 1% L-Glutamine, and 1% sodium pyruvate. Mature, adherent cells were collected on day 7 after harvest and plated in 6-well plates at 5×10⁵ cells/well. Two days later, cells were stimulated with 2.5g/mL of HMW poly I:C (HMW poly I:C; Invivogen #tlr3-pic), 25ng/mL of R848 (Resiquimod, Invivogen), or 5g/mL of LPS (List Biologicals; #201). Cell supernatants and cell lysates were collected at the indicated time-points.

• Product #201 – LPS from Escherichia coli O111:B4

… At the same time and also at day 2 after immunization, 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA) was intraperitoneally administered. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Mice were intraperitoneally injected with 400 ng of pertussis toxin (List Biological Laboratories Inc., USA) at the same time of immunization and again 48 h later. EAE mice were divided into 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Retrograde labeling of RGCs

RGCs were retrogradely labeled following a previously described procedure.17, 18 Briefly, after rats were deeply anesthetized with 10% chloral hydrate (4 mL/kg), 1% cholera toxin B subunit (CTB, List Biological Laboratories, Campbell, CA, USA) was injected into the bilateral superior colliculus (2 µL each site, 6.0 mm posterior and 2.0 mm lateral to the bregma, 5 mm deep from the cortical surface). Rats were killed after 5 d of survival and an anti-CTB antibody was used to detect CTB labeled RGCs. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Test Substances:

… Clostridium difficile Toxin A (ToxA) was purchased from List Laboratories, Inc. (Campbell, California, USA) 

Exposure of T84 Polarized Intestinal Monolayers to proteins: BSA, WGA, ToxA, SLO as well as () control (media) & (+) control (TX-100) for 24, 48, and 72 h. (A) TEER measurement (B) FITC-inulin flux …

• Product #152C – Toxin A from Clostridium difficile

For mice:

We have developed highly sensitive retrograde labeling of facial motor neurons for mice along with CMAP recordings. This method not only labels motor neurons, but also sensory afferent fibers to the trigeminal nuclei [21]. Materials are listed in Table 4.

1st antibody Reagent List Biological Lab 703 Goat anti-CTB

Staining in free-floating configuration (room temperature, light shielded):

1.  10% NRS in PBS-XCR, 30 min on shaker.
2.  1:10,000 dil. Goat anti-CTB (703, List) in PBS- XCR, overnight on shaker.
3.  PBS-X wash (quick 1, 10 min 2). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

MATERIALS AND METHODS:

C. difficile Toxins: C. difficile toxins were obtained from List Biological Laboratories (Campbell, CA) days post-plating) were treated with purified C. difficile toxin A (152C, List Biological …

• Product #152C – Toxin A from Clostridium difficile

… Diphtheria toxin (List labs) at 10 ng/g body weight was administered intranasally at P18 and P22. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Pertussis toxin was purchased from List Biological Labs (Campbell, CA) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)