Citations

Neutralizing Antibody Assay:

Toxin neutralizing properties of antiserum were determined using Vero cells and C. difficile purified toxin B (List Biological Labs, Campbell, CA, United States).

• Product #155 – Toxin B from Clostridium difficile

…Endotoxin-free, ultrapure lipopolysaccharide (LPS) was purchased from List Biologicals (Campbell, CA). …

Author specified the following ultrapure LPS used in their research:

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

… Human tumor necrosis factor-alpha (TNF-), interleukin (IL)-1, IL-6 (Peprotech, Rocky Hill, NJ) and lipopolysaccharides (LPS; List Biological Labs, Campbell, CA) were used for the pro-inflammatory cytokine stimulation, in FBS-free MEM. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Adjuvants:

…The cholera toxin (CT) adjuvant used was the B subunit of CT, pre-clinical grade (List Biological Laboratories, USA), supplied as lyophilised powder and reconstituted according to the manufacturers instructions. The adjuvant was mixed with the viral vectored vaccine by gentle pipetting and administered at doses and routes indicated in the Results and the Figures.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction and monitoring of EAE:

…EAE was induced in 8- to 12-week-old female hCCR6-Tg/mCCR6^/ mice by immunization with 75 g of recombinant rmMOG emulsified in Complete Freund Adjuvant (CFA, Sigma-Aldrich) containing 4 mg/ml Mycobacterium tuberculosis (Difco) subcutaneously on the inner side of both hind flanks. Mice were given 350 ng of pertussis toxin (List Biological Laboratories) i.p. on day 0 and again 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

Active EAE was induced in C57BL/6 female mice by subcutaneous (s.c.) injection of 200 g myelin oligodendrocyte glycoprotein (MOG)3555 peptide (United Biochemical Research, Seattle, WA, USA) in 50 L of complete Freunds adjuvant (CFA) containing 5 mg/mL Mycobacterium tuberculosis H37Ra (Difco Laboratories, Detroit, MI, USA). Mice also received intraperitoneal injections of 400 ng Bordetella pertussis toxin (PTx) (List Biological, Campbell, CA, USA) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mouse breeding and treatments:

…DT (List Biological Laboratories Inc.) was injected i.p. at 10 ng/g of body weight every 48 hours for a total of 4 injections. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

…For CTB injections, a glass micropipette … filled with 1% low salt CTB (List Biological Laboratories, Campbell, CA) in distilled water was stereotaxically … 4°C, and then in TBS containing goat anti-CTB (1:100,000; List Biological Laboratories …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

…in 6 week- old mice subjected to a sensitizing protocol consisting of the oral administration of the amount equivalent to 5 mg of protein per mouse and 10 µg of cholera toxin (CT, List Biologicals, Campbell, CA, USA) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

LPS transfection:

LPS transfections were performed as previously described (Hagar 2011), with modifications. Briefly, 100 ng of rickettsial LPS or Salmonella LPS (List Biologicals) was added to Opti-Mem (Gibco), and 50 ng of Lipofectamine 2000 (Invitrogen) or DOTAP (Thermo-Scientific) was added to Opti-Mem and allowed to equilibrate for 5 min at room temperature. The LPS and Lipofectamine/DOTAP mixtures were combined and allowed to form complexes at room temperature for 30 min. Cell culture medium of BMM were replaced with Opti-Mem, and the LPS-transfection reagent complexes were added. Cells were centrifuged at 300 x g for 5 min, and then incubated at 4 hours at 37C. Cell medium was replaced with DMEM/F12 and 10% v/v fetal bovine serum for 20 hours. Cell lysates and medium were collected after a total of 24 hours post-transfection.

Author did not specify which List Labs Salmonella LPS was utilized.  List Labs provides the the following Salmonella LPS:

• Product #225 – LPS from Salmonella typhimurium
• Product #304 – LPS from Salmonella minnesota R595 (Re)
• Product #434 – ULTRA PURE LPS from Salmonella minnesotaR595 (Re)