Citations

EAE:

… Mice were immunized with a MOG 35-55 kit (EK-2110; Hooke Laboratories). Each mouse was immunized with a 20-L sc injection of MOG 35-55 /CFA emulsion and an ip injection of 200 ng of pertussis toxin (List Biological Laboratories) diluted in sterile PBS (14). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE disease model:

The experimental autoimmune encephalomyelitis (EAE) model was used as previously described⁴⁵. Briefly, 7-8 week old female mice were immunized s.c. with 200g MOG₃₅₅₅ (Life Technologies) in complete Freunds adjuvant (Difco). Mice were given 400ng pertussis toxin (List Biological Labs) in 500l PBS i.p. on day 0 and 2 post-immunization. For adoptive transfer, 110⁶ WT Foxp3 (GFP⁺) iTreg cells or PD-1HKO Foxp3 (GFP⁺) iTreg cells were injected i.v. before immunization on day 0. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…Mice were injected iv with 300 ng of reconstituted lyophilized Pertussis toxin (List Biological Laboratories) on the day of immunization and two days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…The MDS formulations were prepared with the following parameters: Matrix materials: poly (dl-lactide-co- glycolide) and poly (dl-lactide) Microsphere mean diameter range: 6-20 m RPA of anthrax (from List Biological Laboratories, Campbell, CA, US …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Pertussis-Toxin List Biological Laboratories, Campbell, CA, USA …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

The following inhibitors were used: PTX (List Biological Laboratories, 100 ng/mL), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tracing of myelinated afferent fiber projections to the dorsal horn:

The transganglionic tracer, cholera toxin B-subunit (CTB), has been used to map the central projections of cutaneous myelin primary aerents in the spinal dorsal horn of rats.25 After six weeks of RTX injection, CTB (1%, List Biological Laboratories, Campbell, CA) was injected into left sciatic nerve of vehicle-, RTX-, RTXMDL28170-, and RTXPEG400/DMSO-treated rats to determine the eect of MDL28170 on RTXinduced sprouting of myelinated aerent bers labeled by CTB in the dorsal horn. The sciatic nerve was exposed at the level after the rats were anesthetized with 10% chloralic hydras (3.5ml/kg, intraperitoneal). Four microliters of CTB tracers were loaded into Hamilton Yuan et al. 3 microsyringes and injected into the sciatic nerves on the left side of the body. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE:

…On days 0 and 1 post-challenge, mice received 400 ng of Bordetella pertussis toxin intraperitoneally (i.p.) (List Biological Laboratories, Campbell, CA; provided with Hooke Kit TM for EAE Induction) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Serology:

…Toxin-neutralizing assay (TNA) was performed as published earlier [53] to qualitatively assess the ability of antibodies generated to neutralize lethal toxin. Briefly, heat inactivated individual sera were serially diluted in 8 log₂ steps with a starting dilution of 1:50 on sterile 96 well plates (BD Biosciences, Heidelberg, Germany) seeded with 10⁵ J774 A.1 macrophages per well. Lethal toxin (LF) was added at concentration of 500 ng/mL PA and 100 ng/mL LF (List Biological Laboratories, Campbell, CA, USA) and survival of macrophages was visualized through MTT (5 mg/mL, Alfa Aesar, Karsruhe, Germany), which is degraded by living cells to a measurable (OD540_nm) colour compound. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Determination of mAb affinity by surface plasmon resonance:

The affinities of mAbs were determined by surface plasmon resonance (SPR) on a BIAcore T200 instrument (GE Healthcare, Uppsala, Sweden) by carrying out two independent multi-cycle kinetic measurements for each antibody. All analyses were performed at 25°C on a CM5 sensor chip in running buffer (10 mM HEPES, 150 mM NaCl, 3 mM EDTA, 0.05% Tween 20). Polyclonal anti-mouse IgG antibody was covalently conjugated to a CM5 sensor chip according to the manufacturers protocol (Mouse Antibody Capture Kit, GE Healthcare, Uppsala, Sweden). Individual mAbs (1 µg/mL) were bound to this mouse capture chip for 30 s at 5 µL/min. Each captured antibody was subjected to five consecutive threefold serial dilutions of epsilon toxin (ranging from 2.47 to 1800 nM; List Biological Laboratories) for 120 s at a constant flow rate of 30 µL/min to obtain a maximum signal of approx. 30 resonance units (RU). …