Bacterial Toxin Research Citations

Methods:

C57BL/6 mice were transcutaneously immunized with 1µg of PspA and 2µg of cholera toxin (CT) six times at weekly intervals and compared with transcutaneously treated controls (PBS alone/PspA alone/CT alone). …

… Cholera toxin (CT) (List Biological, Campbell 

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE:

in the flanks with 200 µg of MOG 35-55 peptide emulsified in 200 µl Complete Freund’s Adjuvant (CFA) containing 5 mg/ml heat-killed Mycobacterium tuberculosis H37RA (Sigma-Aldrich) and ip injection with 200 ng pertussis toxin (List Biological Laboratories, Campbell 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

All animals received pertussis toxin (PT) (100 ng per mouse ip; List Biological Laboratories, Campbell, CA) on days 0 and 2 after the first immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… 100 µg pure MOG 35-55 in complete Freund’s adjuvant (CFA, 8 mg/mL Mycobacterium tuberculosis, M. tb) (IFA from Thermo Fisher and M.tb from BD, Sparks, MD) was injected into the abdomen subcutaneously on day 0. Pertussis toxin (List Laboratories, Campbell 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis induction and ERβ-ligand treatment

Female mice were immunized subcutaneously with MOG, amino acids 35–55 (200 μg per mouse, Mimotopes) emulsified in Complete Freund’s Adjuvant, supplemented with Mycobacterium tuberculosis H37Ra (200 μg per mouse, Difco Laboratories), over two sites drained by left inguinal and auxiliary lymph nodes in a total volume of 0.1 ml per mouse (Day 0). Pertussis toxin (500 ng per mouse, List Biological Laboratories) was injected intraperitoneally on Day 0 and Day 2. On Day 7, a booster immunization was delivered over contralateral lymph nodes. Ages of female mice for EAE induction were 8 to 12 weeks.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Chemicals, reagents, equipment and safety:

… Recombinant LF (rLF) was purchased from List Biological Laboratories (Campbell, CA).

Sample preparation:

The MS method for anthrax LF relies on three simple steps that each add a layer of sensitivity and specificity (Fig. 1). LF-MABs selectively purify and concentrate total-LF (LF + LTx) (Step 1). Immobilized LF is reacted with an optimized peptide substrate which it hydrolyzes producing two unique LF-specific product peptides (Step 2). Because each LF molecule can continuously hydrolyze peptide substrate, this step allows amplification of the resulting signal and improves detection. Analysis of the cleaved peptides by high-sensitivity/high-resolution isotope-dilution (ID) MALDI-TOF MS (Step 3). This strategy has been successfully applied to several enzymatic protein toxins [22].

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

Cell culture and treatment:

… For stimulation, cells were exposed to 100 ng/ml LPS (ultrapure smooth LPS from E. coli O111:B4,
List Biological Laboratories, Campbell, CA).

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Localized EAE Mouse Model:

Adult female C57BL/6J mice (68-weeks-old) were subcutaneously immunized with an emulsion of 50 g myelin oligodendrocyte glycoprotein (MOG)3555 … All mice received an intravenous injection of 200 ng pertussis toxin (List Biological Laboratories) in 100 µL PBS at 48 h after injection of TNF- and IFN-. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction. Mice were s.c. injected into both flanks with a total of 200 μl of emulsion containing either 300 μg of MOG35–55 (Synpeptide) or 500 μg of bMOG (see above) dissolved in saline and mixed with an equal volume of complete Freund’s adjuvant containing 500 μg of killed Mycobacterium tuberculosis H37 RA (Difco Laboratories). Mice were also i.p. injected with 20 μg/kg of PTX (List Biological Laboratories) immediately and 2 days after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Diphtheria Toxin List Biological Labs 150

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae