Bacterial Toxin Research Citations

EAE:

Female C57/BL6 mice of 810weeks were used. EAE was induced by the subcutaneous application of 50g of myelin oligodendrocyte glycoprotein (MOG) peptide (amino acids: 3555) emulsified in 100L complete Freunds adjuvant (Difco Laboratories) and supplemented with 10mg/mL Mycobacterium tuberculosis, on the side of each hind flank. Pertussis toxin (200L of 0.5g/mL solution; List Biological Laboratories) was injected intraperitoneally (IP) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…To obtain cell culture supernatants containing soluble native IL-2, equine PBMC were either stimulated with PMA and ionomycin as described above but without an inhibitor of protein secretion, or with LPS E. coli O55:B5 at 12.5 g/ml (List Biological Laboratories, Campbell …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Experimental autoimmune encephalomyelitis (EAE):

… In addition, 100 ng/mouse of Pertussis toxin (List Biological Laboratories) were injected i.p. at days 0 and 2 of EAE induction. Mice were monitored daily for clinical disease symptoms according to the following scale: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…three consecutive days on the first week and once per week during the following 4 weeks, with the equivalent of 5 mg of protein per mouse of WP (two groups, n = 6/group) or HWP (two groups, n = 6/group) in PBS, plus 10 µg of cholera toxin (CT) (List Biologicals, Campbell …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE Model:

EAE was induced by s.c. injection of 200 g of myelin oligodendrocyte glycoprotein (MOG)₃₅₅₅ (GenScript) with IFA (Difco) plus 500 g nonviable, desiccated Mycobacterium tuberculosis (Difco) into the hind flank. On the day of and 2 d after immunization, 200 ng of pertussis toxin (List Biological) was injected intraperitoneally. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Subjects and experimental outline:

In total, 40 mice (both genders) were used in this experiment with 20 each assigned to the control (Cre) and experimental (Cre⁺) groups. All subjects in both groups received one intraperitoneal injection (i.p.) of DT at a dose of 25 ng/g (List Biological Laboratories, Campbell, CA, USA) at postnatal day 21. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Toxins:

TcdA and TcdB₍₀₀₃₎ were purchased from List Biological Laboratories …

Murine passive protection:

Female C57BL/6 mice (78 weeks old) were administered 2 mg anti-T-toxin hIgG or PBS IP. Mice were challenged IP 24 h later with a MLD_100% of TcdA, TcdB₍₀₀₃₎, or CDTa plus CDTb and observed for 7 days.

• Product #152C – Toxin A from Clostridium difficile 
• Product #155 – Toxin B from Clostridium difficile

Macrophage culture and activation:

Murine peritoneal macrophages were elicited with thioglycollate (BD, Sparks, MD, USA; same batch for all strains) for 4 days. Cells from up to 4 mice were pooled and plated at 4 10⁶ cellsml¹ in DMEM+20% FBS+1% streptomycin/penicillin in duplicates or triplicates. After overnight culture, adherent cells were selected (adherence assay for macrophage enrichment). RNA deconvolution shows two main populations of inflammatory macrophages (F4/80+, MHCII and F4/80, MHCII+) and a small population of neutrophils (Supplementary Fig. 2). Cells were incubated with 2 ngml¹ LPS (List Biological, Campbell, CA, USA) or control in DMEM+1% FBS for 4h before harvest. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Induction and clinical assessment of EAE:

…Mice were immunized subcutaneously in both sides of the flank with 200 g MP4 in 200 l CFA. Mice received intraperitoneal injections of 200 ng pertussis toxin (List Biological Laboratories, Hornby, ONT, Canada) on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Mice were immunized with a MOG 35-55 kit (EK-2110; Hooke Laboratories). Each mouse was immunized with a 20-L sc injection of MOG 35-55 /CFA emulsion and an ip injection of 200 ng of pertussis toxin (List Biological Laboratories) diluted in sterile PBS (14). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)