Bacterial Toxin Research Citations

EAE:

… At the same time, mice were also administered 250 ng of pertussis toxin (List Biological Laboratories) ip and again 2 d later. Clinical disease was monitored daily and scored …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Evaluation of anti-CLEC12A antibody in EAE immunized mice:

Progressive EAE was induced in female C57BL/6 mice (68 weeks old) and the CLEC12A/ mice as described previously⁶⁰⁶². Briefly, 100L of 150g myelin oligodendrocyte glycoprotein (MOG₃₅₅₅) (PolyPeptide Laboratories) peptide emulsified in complete Freunds adjuvant (Difco) containing 4mg/ml killed Mycobacterium tuberculosis (strain H37Ra; Difco) was injected subcuataneously. Following immunization, 200ng of pertussis toxin (List Labs) was injected i.p. into mice on Day 0, followed by a 400ng pertussis toxin i.p. injection on Day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Macrophage Culture And Activation:

…After overnight culture, cells were washed in PBS and incubated with 2 ng/ml LPS (List Biological Inc., Campbell, CA, USA) or control in DMEM + 1% FBS for 4h before harvest. Viability of cultured macrophages was determined for some strains by staining with 2 M calcein AM …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Proteins:

…Protective antigen (PA) from Bacillus anthracis (List Biological Labs, Campbell, CA, USA) was used in the assay at a final concentration of 3 g/ml. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Preparation of the MDS formulations:

RPA of anthrax (from List Biological) Loading: 0.8-1%

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

– … After resolving the desorbed Pfs25-EPA on a non-reducing tris-acetate SDS-PAGE gel, Western blot analysis was performed using anti-Pfs25 monoclonal antibodies 4B7 and 1G2 (LMIV) and an anti-Exotoxin A polyclonal antibody (List Biological Laboratories, Inc, Campbell …

• Product #760 – Anti-Exotoxin A (Goat) from Pseudomonas aeruginosa

a-cholera toxin B, List Biological Laboratories; Campbell, CA, goat, 1:2000

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Mice, BM chimeras and in vivo treatments:

…For experiments with Ccr2-depleter mice, mice were injected ip with 10 ng/g Diphtheria Toxin (DT) (List Biological Laboratories) in PBS. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… For the retrograde tracing, 0.2 µl 0.4% CTb (#104, List Biological Laboratories, Campbell, CA, USA) were injected at the following coordinates for the VS: + 2.2 mm anterior to Bregma; 0.75 mm lateral to the midline; and dorsoventral 4.25 mm below the dura. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

…As indicated, the pipette solution was added with ATPS (EMD Millipore Chemicals, Germany), the light chain of botulinum toxin C (List Biological Lab., USA), Pitstop 1 (Abcam, USA), an AP2-blocking peptide containing the DNF motif of …

• Product #625A –  Botulinum Neurotoxin Type C Light Chain, Recombinant