Bacterial Toxin Research Citations

… Ultra-pure LPS (Escherichia coli 0111:B4) was obtained from List Biologicals (Campbell, CA, USA). … Ultra-pure LPS (E. coli0111:B4) from List Biologicals was dissolved in DNase-/RNase-free sterile normal saline and intraperitoneally injected (5 mg/kg) (44, 46). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Induction of EAE and Treatment:

…Mice were immunized s.c. in the flank with 200g MOG_35-55 emulsified in CFA supplemented with 300g Mycobacterium tuberculosis (Mt) H37RA (Difco). Pertussis Toxin (PTX, List Biological Laboratories, CA, USA) was injected i.v. at the time of immunization and 48h later. In some experiments, animals were sacrificed prior to onset of clinical symptoms in order to analyze the lymph node response. EAE was scored on a scale of 06: 0, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

EAE was actively induced by subcutaneously injecting 250 L MOG₃₅₅₅ peptide… Additionally, the animals received intraperitoneally (i.p.) 200 ng pertussis toxin (List Biological Laboratories) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tracer Injections:

… For retrograde tracing, a low salt formulation of Cholera Toxin Subunit b (CTb; No. 104, List Biological
Laboratories, Campbell, CA) was applied via iontophoretic injection (Ericson & Blomqvist, 1988). …

Immunohistochemistry:

… For visualization of CTb, the tissues were washed three times for ten minutes in PB and then
incubated overnight at 4°C in a primary antibody solution of goat antiserum against CTb (List Biological
Laboratories, Campbell, CA) and 0.3% Triton PB. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Induction of EAE:

The MOG mouse model of EAE is the preferred model of MS … Animals received two intraperitoneal (IP) injections of pertussis toxin (PTX: List Biological Laboratories; #179B) (0.2 µg in 100 µL of PBS at Days 0 and 1) to open up the blood brain barrier and facilitate the entry of pathogenic T cells to the CNS. AC mice received all the same treatment as the EAE mice with the exception of the omission of the MOG antigen. …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice were orally administered PBS or 50 mg of Ovalbumin (OVA) (Sigma-Aldrich) with 10 μg of cholera toxin (CT) (List Biologicals, Campbell, CA, USA) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

…female SftpcCreERT2 +/ ;HIF1 f/f mice (or littermate controls lacking one of the transgenes), aged 8 to 12 weeks, were treated with 2 g/g body weight lipopolysaccharide (LPS) (Escherichia coli 0111:B4; List Biological Laboratories, Campbell …

• Product #201 – LPS from Escherichia coli O111:B4

…Cells were stimulated with lipopolysaccharide (LPS) (Escherichia coli O111:B4, List Biological Laboratories) at 10ng/mL or 30% bronchoalveolar lavage fluid (BALF) pooled from nine patients with ARDS diluted in RPMI-1640 1% FBS for 24 or 72 hours. …

• Product #201 – LPS from Escherichia coli O111:B4

Immunization procedures:

Peptide emulsions were prepared in CFA containing Mycobacterium tuberculosis H37RA extract (5mg/ml, Difco Laboratories, Detroit, MI). Mice were immunized with these emulsions subcutaneously on days 0 and 7. Animals also received PT (List Biological Laboratories, Campbell, CA; 100ng/mouse) intraperitoneally (i.p.) on days 0 and 2 after the first immunization [21]. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE and anti-CD3 treatment:

Active EAE immunization with MOG35-55 peptide (Gene Script) emulsified in complete Freund’s adjuvant (CFA) (Difco) along with pertussis toxin (List Biological Laboratories) injections …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)