Bacterial Toxin Research Citations

…They were then incubated overnight in a primary antibody against CTB (anti-cholera B subunit made in goat, List Biological Laboratories, CA# 703, antibody RRID AB_10013220, Lot# 7032A6) diluted 1:33,000 in a blocking solution consisting of 0.4% triton and …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Antibody Determination:

Pertussis toxin, FHA, and Prn were purchased from List Biologicals and used to coat 96-well plates (Nunc) at 1 g/well overnight at 4C in PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free). 

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

Product #170 is currently out of stock.  Large quantities may be obtained through Bulk Requests and Customer Orders.

Reagents and mouse strains:

…Pertussis toxin was purchased from List Biological Laboratories.

EAE induction following the fecal transfer:

EAE induction was performed as described previously… Briefly, on day 0, mice were immunized subcutaneously with 150 g of MOG₃₅₅₅ peptide emulsified in CFA in the two hind flanks and received 200 ng of pertussis toxin intraperitoneally 2 h later. Mice received another 400 ng of pertussis toxin on day 2 of the immunization…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

 …LPS was obtained from List Biological Laboratories (San Jose, CA, USA)…

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE:

On the day of immunization and 48 h later, mice were administered intravenously with 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA, USA) in 200 l of PBS. Mice received daily intraperitoneal …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… incubated for 24 hours at room temperature in goat polyclonal anti-cholera toxin B subunit (1:1000, List Biological Laboratories, Campbell, CA; cat#7032A6; RRID: AB_ 2313637), rabbit … against recombinant Cholera toxin B subunit (List Biological Laboratories, Campbell …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Author did not specify which CTB was utilized.  List Labs provides Product #103 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae) and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

Test substances:

…and Clostridium difficile toxin A (ToxA) was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA).

Experimental design:

Each experiment involved 48 individual primary human small intestinal epithelial barriers that were split into two groups (group #1 and group #2) and analyzed in parallel. …TEER was measured for each epithelial barrier in duplicate following equilibration as described above (0 h), followed by exposure to differing concentrations of test proteins (BSA or ToxA), …Group #1 and group #2 were each split into 8 subgroups of three intestinal epithelial barriers apiece and treated with 100 l assay media alone, 0.1 g/ml TNF-/FliC, 100 g/ml BSA, 1000 g/ml BSA, 0.02 g/ml ToxA, 0.2 g/ml ToxA, 2 g/ml ToxA, …

Product #152C – Toxin A from Clostridium difficile

Abstract:

…We studied the axonal transport of cholera toxin subunit B to investigate the central projections of this network in order to identify its function. …

Methods:

…Five microliters of 1% CTB (List Biological Labs, Campbell, California, USA) solution was soaked into the filter paper.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

…Pertussis toxin was purchased from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Introduction:

…This study examined the role and mechanism of HDAC8 in adaptive responses of the human monocytic THP-1 cells to LeTx-induced cell cycle arrest. We found that HDAC8 played a pivotal role in activating the PI3K-AKT signaling axis in LeTx-exposed cells through inducing histone H3 lysine 27 tri-methylation (H3K27me3) and subsequent inhibition of PTEN expression.

Reagents:

PA and LF were purchased from the List Biological Laboratories (Campbell, CA, USA).

Lethal Toxin consists of Lethal Factor & Protective Antigen:

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence)

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis